Analysis of Aromatic amines

benzocaine, procaine hydrochlorideparacetamol, lidocaine hydrochlorideadrenaline, dopamine hydrochlorideoxprenolol, carteolol hydrochloride

Contents

(Feature)2. BABAPABA3. 4. 1. Schiff

1. 3. 4. 5. (Feature)2.()+/

--Identification

1.2.Identification

Identification

/ /Identification

a. b.Identification

10NaOH()Identification

UVIdentification

Identification

IR cm-1 cm-1 C=O 1692 C-O 1271, 1170, 1115 N-H 2585 2645 1550 N-H 1280 N-H Identification

Identification

Detection of specific impurities11 2pH5.5-6.5(1

Detection of specific impurities2.3.O-TLC(254nm)

Detection of specific impurities4. 1.0g1220ml1ml30min1.0g50g0.005%

Detection of specific impurities1.2%TLC

Detection of specific impurities (ropivacaine) SR

Detection of specific impurities

Assay (volumetric analysis) ( spectrometry ) ( chrometography ) (sodium nitrite method)(non-aqueous titrimetry)

Assay(sodium nitrite method)NaNO2 + HCl HNO2 + NaClHNO2 + HCl NOCl + H2OHBrNO+ HBrNO+

Assay11030HNO2 [Ar-NN]Cl- + H2O Ar-OH + N2+ HCl2 3NaNO2,42/3HNO25KBr6(-)

Assay(non-aqueous titrimetry)

AssayUV257nm2005

AssayHPLCHPCE

(Phenethylamine)

*

Identification

Adrenaline0.1mol/LNoradrenaline bitartratePhenylephrine hydrochlorideIsoprenaline hydrochloride5%Dopamine hydrochloride1%

Identification

IdentificationUVIR

(mg/ml)nm (nm) (A)0.102720.052800.500.5%0.032800.1mol/L0.25263, 2700.012340.600.1mol/L0.03243, 2960.082740.50251, 257, 263

IdentificationChp 10mg1ml220%1ml1ml

Detection of specific impurities

Detection of specific impurities

Detection of specific impurities

C(mg/ml)(nm) A HCl(92000) 2.0 3100.05 2.0 3100.20 2.0 3100.05 2.0 3100.15 1.5 3470.06

Detection of specific impuritiesHPLCTLC

Assay

Assay()

Assay2

Assay()8ml 0.05mol/L25ml20.1mol/L1ml0.05mol/L18.42mgC18H26N2H2SO4

AssayUV HPLC (GC-MS)

(enalapril maleate)(benazepril hydrochloride)(lisinopril)

(lisinopril)

Identification

Detection of specific impurities

Assay(Ch.P)(BP)-HPLC

Assay