antibiotic19441950s1980s

1990smicrobial medicine

50352011.4.7WHO20012011FDA3/12 30GSKAstraZeneca

NMRBBP/ChP93.0%USP85.0%A3+B+A1A1HPLCUVIRNMRMS

TLCHPLC

A-78C-65-62ChP-78-71D()L()48

ChP6.0%3.0% 3.0%

-490nmA

5150m

ICHChP

-2-2-GC13-2-0.3%0.8%33%

----2mg/day4g-100 ppmChPHPLC

USP1990 GGG

50m

96HPLC5

HPLC-UVHPLC-ELSDChP HPLC--UVHPLC-ECDBPUSPGCBP BP USP

-

Sephadex G10--

Superdex Piptide VTSK 2000SWXL ChP:

TSK2500 PWXL TSK Gel G2500 HXL- cefcapene prvoxil hydrochloride

/

water activity

N- BPUSPJPIC ChPtITP-CZEIC ICNMPNMP+

N-ICNMP + NMP10%

N-tITP-CZEtITPNMP + pH4.74~7CZE0.003%0.01%

MEKCHPLC(ChP)AHPLCTLCHPLCCEHPLCHPLCHPLCHILICTLCGCCE(EP/BP)

MEKC

Offline-2D-HPLC-CE

RP-IP-HPLC-PED

RP-IP-HPLC-ELSD

Analysis of underivatized gentamicin by CE-UV detection

precapillary-derivatization-MEKC

EMMA-CZEprecapillary-derivatization-CZE

EMMA-CZEprecapillary-derivatization-CZE

CZE-C4D

1969

HPLC

0

2

4

6

8

10

12

14

16

Time [min]

0.0

0.5

1.0

1.5

2.0

2.5

6

x10

Intens.

EIC 137 -All MS

EIC 155 -All MS

EIC 200 -All MS

EIC 258 -All MS

EIC 275 -All MS

EIC 396 -All MS

b

d

a

fosfomycin

c

e

tITP-CZE-UV

CZE-UV

/

QbD

ICH

2009

J2011, 46: 928/(QSPR/QSAR)201125710

NMR(2011374241272012.12)USP/BPpH3.55.5ChPpH4.07.0

X-NMR-2009