optical tweezers(광집게 의...
TRANSCRIPT
Optical Tweezers(광집게)의원리와 응용
Optical tweezers?
두 빔에 의한 포획 광학 부상 (optical levitation)
광
섬
유
빛의 산란에의한 힘
중력
대물렌즈
Optical tweezers(광집게)
History and Issues
1970, Ashikin : Optical levitation
1986, Ashikin et al : Optical tweezers
1990, Burns et al : Periodic structures by using interference patterns
1997, Chiou et al : Trapping and manipulation of micro particles
by using interference fringes
…
<Issues>
- Optical force model
- Manipulation(patterning) of multi-particles
- Bio-Medical applications
1. Optical force models
Optical tweezers
Electromagnetic model
Ray Optics model
- For R <<10
- For R >>10
1) Electromagnetic model
The change in the electrostatic energy ;
1
2
dVtrdVW T
VV
),(E2
)()EE)((
2
1 2
112
1212
11
V1
22
2
2
22
2
201
22exp
2)(
2)(
w
z
w
yx
w
PrE
cnrI
dVrIcn
nn
V
1
)()(
1
2
1
2
20
WF
2
0
22
222
0
2
1
2
1
2
20 sinh2
exp2
)(4
w
Rza
w
Raz
w
Raerf
cn
PnnF ccc
ax
2
0
2
222
0
1
2
1
2
20 sinh2
exp22
)(4
w
Ra
w
Ra
w
Raerf
w
Raerf
cn
PnnF cccc
tr
2) Ray optics model
scattering force
gradient force
A B
F
beam axis
1 234
y
x
z
O
O
r
PRP
TRP
T2RP
FA
Fs
Fg
FAF
B
S
Qc
PnF 1
collimated beam
Fg < Fs
focused beam
Fg > Fs
Generally, optical trapping force
Fg : gradient force
Fs : scattering force
- Optical force
(1) Axial trapping
dPqc
ngdF
dPqc
nsdF
gg
ss
1
1
ˆ
,ˆ
where,
2
2
121
2
1
2
2
121
2
1
2cos21
]2sin)(2[sin2cos
,2cos21
]2cos)(2[cos2cos1
RR
RTRq
RR
RTRq
g
s
gsnet FdFdFd
0
0
2
0
22
02
0
)sincos)(/2exp(2
4
sgax qqwrrdrdQ
ax
sgax
Qc
Pn
qqwrrdrdc
PnF
1
0
2
0
22
02
0
1 0
)sincos)(/2exp(2
4
- Axial trapping efficiency
- Axial trapping force
(2) Transverse trapping
IdSqqc
ndF sgnet )cossin(1
tr
sg
Qc
Pn
rrdrdc
PnF
1
0 0
2
0
2
2
0
1
)sincos(
)/2exp(4 0
Experimental setup
Laser
diode
Objctive
lens
lens
PBS
lens1 lens2
CCD
Dichroic
mirror
Hallogen lamp
Z-axis
Y-axis
Motorized translation
stage
Trapping Image according to trapping direction
Axial trapping
transverse trapping
논의점 - 광포획 효율의 변화 (포획 깊이)
Laser
diode
Lens 1Lens 2
Dichroic
mirror
chamber
Cover glass
Slide glass
Index matching oil
R
(aperture size)
Beam radius
stage
Objctive lens
논의점 - 광포획 효율의 변화 (빔반경)
1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6
0.005
0.010
0.015
0.11
0.12
0.13
0.14
0.15
0.16
0.17
Q
ratio (0 / R)
For small particle
For big particle
3) Beam optics model
2
0
2
0
2
0
2
0 1
z
r
2
0
2
0
2
0
22
0
2
0
2
0
2
0
11
z
rz
rzR
2
0
2
0
2
0
2
0
z
rzz
The calculation for existing model was performed the fixed incident angle for all
particle. That is, incident angle independent of particle diameter.
2
0
2
0
2
0
22
0
2
0
2
0
2
0
2
0
2
0
2
0
2
0
11
1
z
rz
rz
z
r
ArcSinR
ArcSin
Incident angle
2. Manipulation(patterning) of multi-particles
CNT
SWNT
( single-walled cabon nanotube)
MWNT
(Mulit-walled cabon nanotube)
1) Carbon nanotubes
CNT trap실험 장치도
Lens 1
Laser
diode
Dichroic
mirror
Lens 2
Cover glass
Slide glass
Index matching oil
Objctive lens
CCD
Hallogen lamp
PC
5m
HY letters
X
Y
5m
X,Y letters
CCD image of aligned CNT SEM Image of ring pattern
시간에 따른 CNT Ring Pattern 형성
00:03
00:10
22:3000:05
01:3000:07
00:00
Laser On 5m
Laser Off
Sequential CCD image of CNT to form ring pattern in the chamber
- OPTICAL TWEEZERS: MICROBUBBLES AND NANOTUBES
Dr. Phil Jones , 6 March 2007
- Nanofabrication with Holographic Optical Tweezers
Pamela Korda, Gabriel C. Spalding, Eric R. Dufresne, David G. Grier
November 16, 2000
- Single-particle microelectrophoresis with optical tweezers
Van Heiningen, Hill, 2007
2) Micro-patterning
Surface tension-enhanced optical
trapping for lateral close-packing
Cell-to-cell interactions
Cell fusion dynamics
1) Raman spectroscopy
RayleighScattering
RamanScatteringE
3. Bio-Medical applications
0
0
0
Rayleigh Stokes Anti-Stoke
m
0 m
0
Schematic Diagram of Experimental Set-up
objective
lens
CCD
camera
chamber
dichroic
mirror
beam-
splitter
LD
monochromater
PC
PMT
grating
dichroic
mirror
source : 0 = 834 nm
The McCreery Research Group(The Ohio State spectrum)
LTRS system 상용 Raman microscopy
5 m
LTRS system 상용 Raman microscopy
m2
포획 깊이에 따른 Raman peak 크기 변화
(1) (2)
2) Yeast trap and manipulation
PC
Laser
CCD
OL
L L
M
M
M
M
L : Lens
M : Mirror
BS : Beam Splitter
OL : Objective lens
BS
Michelson
interferometer
광 포획된 yeast의 발아
발아 하고 있는 yeast
발아하고 있는 yeast의 딸세포에 대한 주위 세포들의 영향
Yeast의 딸세포의 발아.
(a) Living yeast cells (b)Dead yeast cells
Changan Xie et. al. Optics Letters 27, 4, 2002
Tying a knot in the protein actin. By using optical tweezers
to manipulate the ends of the molecule, the chain is curl..)
3) Biological applications
Calibration of optical trapping efficiency
Dependence of the optical trapping efficiency of
a biconcave red blood cell flowing in a rectangular
microchannel on the sphericity index and the channel geometry
Applicational example) Malaria-infected RBC
Combined microchannel-type erythrocyte (RGB) deformability test with optical tweezers
Cell damage during femtosecond optical trapping
Damage thresholds for biological cells(RBC)
Platelet(혈소판)
RBC-under threshold
RBC-above threshold
6 mW 20 mWLaser power : 90% of the damage threshold (1.8 mW)
t=0 t=7m 30s
t=13m 5s t=20m
t=30m
damage
scar
Cell disruption