phcc biology new facutly orientation manual

New Faculty Orientation Manual Biology Department

Natural Sciences – Pennsylvania Highlands Community College 101 Community College Way Johnstown, PA 15904 - (814) 262-6400 - www.pennhighlands.edu

Pennsylvania Highlands Community College 2

Index

Welcome…………………………………………………………………………. 3

Frequently Asked Questions …………………………………………………… 4

Lab Classroom / Storage Tutorial …………………………………………….. 6

Storage and Prep Rooms ………………………………………………………. 7

Floor Plans ……………………………………………………………………… 9

Biology Course Descriptions ………………………………………………… 11

Stocked Lab Supplies Listed by Course ……………………………………… 14

Lab Resources Location by Room …………………………………………….. 17

Purchase Orders ……………………………………………………………….. 25

Protocol ……………………………………………………………………….. 26

Lab Safety ……………………………………………………………………… 27

Additional Biology Resources ……………………………………………… 32


Welcome We want to first welcome you to the Biology Department, here at Pennsylvania Highlands

Community College. This packet includes information that will be useful in your faculty role as an

instructor.

This is designed to make your transition into the Biology Department at Pennsylvania Highlands

Community College more pleasant and productive.

The Biology department provides a solid foundation in science by instilling in students a

commitment to search for truth through application of the scientific method, to gain a better

understanding of humans, the earth and the universe, and to desire knowledge that can be applied

to the service of others. The department offers courses that fulfill the college's Core Curriculum, as

well as courses that support the Biotechnology Program.

Your role as a member of the department is to aid our endeavors of creating a learning

environment that enhances critical thinking, allows for collaboration, and exploration through

experience so that we can impart life long learning skill on our students.

We hope you have a great semester!

Ashley Hollern Jill Mitchell

Quick Contact List for New Faculty

Name Title Contact

Jill Mitchell Instructor, Biological Sciences Lead Faculty 262-6487

Ashley Hollern Instructor, Biological Sciences 262-6412

Important Phone Extensions

Security – x 6247

Security Emergency – x 5555

IT Help Desk – x 6470

Front Desk – x 6400

To make a local call from any room

dial 9 first.

http://www.youtube.com/watch?v=45bT9dmV9-A&feature=youtu.be


Frequently Asked Questions

1. How do I obtain the keys for my classroom?

The keys for your lecture are lab room can be obtained from Mary Ann Lee during the

hours of 8:30am and 4:30pm. If your class is held after these hours you should contact

security at ext. 6427, which can be dialed from any open classroom or from the faculty

copy room.

2. Who are the full time departmental employees in biology?

Jill Mitchell is the Biology Department lead faculty. Jill teaches BIO110, and BIO115.

Her office is located in C. Ashley Hollern is a full time biology faculty member and

teaches BIO210, BIO260, and BIO262. Her office is located in C202.

3. Who can I ask for assistance while teaching lecture / lab?

Feel free to contact either Jill Mitchell at ext. 6487 or Ashley Hollern at ext. 6412 with

any questions or concerns regarding lab or lecture questions.

4. What supplies are available to me?

You will find a list of supplies available to you in this manual.

5. Is it ok to borrow supplies from another lab cabinet?

We would prefer you obtain the lab supplies from the lab cabinet assigned to your class.

However, if supplies are in short supply and you must borrow supplies from another

cabinet, please make a note of the need for a purchase order. There is a tablet for

“supplies needed” that can be found in C230B Storage room, or C226 B Storage room.

6. Where do I make copies?

Copies can be made in the faculty copy room located in C224.

7. Who do I contact if I need additional supplies?

If you are aware of the need for additional supplies at the beginning of the semester, you

should contact Jill Mitchell with the Supply list, company name and purchase order

information so that the order may be processed in a timely manner.

8. How do I address supplies that I run out of during the semester?

If you should run out of supplies during the semester and no replacements are available,

contact Jill Mitchell ASAP so that an order may be processed.

9. What is the proper PHCC procedure for disposing of laboratory material?

You will find a “Lab Safety” section in the manual outlining the proper procedure of

handling hazardous materials handled during the semester.

10. Where can I find manuals to the lab equipment available to me?

The lab equipment manuals can be found in the C230 A Prep Room, located in the

drawer beneath the incubators labeled “Equipment Manuals”

11. How do I address Lab Safety in Lab?

At the start of the semester, students should be made aware of proper lab safety protocol.

You are required to show a brief video on “Lab Safety” from Flinn Scientific. Following

the video ALL students are to receive the “Lab Safety Contract” that they must read and

sign, prior to the start of any lab. Please keep the contracts until the end of the semester,

at which time you must turn them into Jill Mitchell.

12. The lab stations are out of lab disinfectant, where can I find more?

The lab countertop disinfectant can be found in the C230 prep room to the left of the

dishwasher in a cabinet marked “Lab Disinfectant.”


13. Where the emergency gas shut-off located in the lab rooms?

The emergency gas shut off is a large red button located near the door. By pushing the

button in, the gas is shut “off”.

14. What is the recharging procedure for the chargeable microscopes located in C224/

C226? Microscopes that have been used should be plugged into the outlet strip and the timer

knob turned. The timer will turn the charge off automatically after 8 hours. Microscopes

that were not used should not be plugged in during this process. Overcharging of the

scopes will shorten the life of the battery.

15. Usage of the dishwasher for glassware? What is it primarily used to clean? It can be

used to clean glassware, but it is best to have students wash glassware at the end of lab so

that glassware remains available to all labs and isn't hanging out in the dishwasher.


Lab Classroom / Storage Tutorial

Click on the image above to view a tour of the lab classrooms and storage areas of the

Richland Campus.

http://www.screencast.com/t/p1GLCcg69W


Storage and Prep rooms

BIO 260 Anatomy Lab Storage/ Prep Rooms

C226B Storage

C226A Prep

The Prep and Storage rooms above are

located between Lab classrooms C224 and

C226. The storage and prep rooms

contain the materials and supplies for the

labs taught in C224 and C226.


BIO 262 Anatomy Lab Storage/ Prep Rooms

C230A Prep

C230B Storage

C230 Prep Room and Storage room is located between C228, and C230. The Prep and Storage

rooms contain materials and supplies for BIO110, 115, 210, and 262.


Floor Plans

7. Microscope Slides 8. Media 9. Autoclave Materials 10. BIO 210 Microbiology 11. Cooler – Cultures/Dissections Specimen 12. Refrigerator – Media/Consumables 13. Grocery Items 14. Laboratory Equipment 15. Biology Club 16. BIO 110 Principles of Biology I 17. BIO 115 Principles of Biology II 18. BIO 210 Microbiology 19. BIO 262 Advanced Anatomy and Physiology II

1. Fume Hood 2. Autoclave 3. Incubator/Autoclave Materials 4. Incubator/Equipment Manuals 5. Sink/Dishwasher 6. Disinfector/Cleaning Supplies

1. Microscope Cabinet 2. Microscopy Kits 3. Countertop Scales/Glassware 4. Gloves


Biology Course Descriptions

BIO 110 - Principles of Biology I

This course provides an overview of the basic principles of biology including the structure and function of the cell, cellular respiration, photosynthesis, mitosis, meiosis, genetics and evolution. Lectures emphasize human biology and are complemented by discussions that stress critical thinking. Lab experiments are designed to teach basic scientific skills, and to reinforce the topics covered during lectures. This course is designed to prepare students for more advanced courses in biology. 4 credits Text: Biology 8e/ Neil A. Campbell

BIO 115 - Principles of Biology II

This course provides an overview of the basic principles of biology including the

structure and function of animals and plants, including organ systems, reproduction and

the regulation of body systems. The course also provides an overview of the Earth’s

ecosystems and the diversity of life within each ecosystem. Lectures are complemented

by discussions that stress critical thinking. Lab experiments are designed to teach basic

scientific skills and to reinforce the topics covered during lectures. This course is

designed to prepare students for more advanced courses in biology.

Prerequisite(s): BIO 110 Principles of Biology I

4 credits

Text: Biology 8e

Campbell / Reece

ISBN: 0321543254

BIO 210 - Microbiology

This course is designed to introduce students to the fundamental concepts of

microbiology, and the application of those concepts to human disease. Lecture will

include the study of microorganisms, their metabolic processes, and their relationship to

disease. Laboratory work includes culturing, staining, studying, and identifying

microorganisms.

Prerequisite(s): BIO105 General Biology with Lab or BIO 110 Principles of Biology I

4 credits

Text: Microbiology: An Introduction, 10e

Tortora / Funke / Case

ISBN: 0-321-55007-1

http://campbellbiology.com/


http://wps.aw.com/wps/media/access/Pearson_Default/6822/6986311/login.html


BIO 220 - Biotechnology I

Biotechnology I is the first semester course of a two semester sequence that is

designed to introduce the student to basic principles of biotechnology and to familiarize

the student with application of these principles in modern biotechnology settings. Topics

will include exploration of biotechnology careers and research applications, industry

standards for safety procedures, basic biotechnology skills and typical instrumentation

related to the identification, isolation and manipulation of DNA. Prerequisite(s): BIO105 General Biology with Lab or BIO110 Principles of Biology I; and

CHM106 Introductory Chemistry or CHM120 General Chemistry I

4 credits

BIO 230 - Biotechnology II

Biotechnology II is the second semester course of a two semester sequence that is

designed to introduce the student to basic principles of biotechnology and to familiarize

the student with application of these principles in modern biotechnology settings. Topics

will include exploration of biotechnology careers and research applications.

Prerequisite(s): BIO220 Biotechnology I

4 credits

BIO 240 - Bioethics

This course introduces the student to basic bioethics. Theories are discussed in a

primary learning level with beginning application levels. Included are current

controversial bioethical topics of significance in the field of biotechnology and

techniques which enable the student to make justified ethical decisions.

3 credits

BIO 260 - Human Anatomy and Physiology

This course introduces the student to the basic structure and functioning of the human

body. This is a semester long introduction to Anatomy and Physiology. Course topics

will include the organization of the body at the molecular, cellular, and tissue levels and

homeostatic mechanisms associated with the endocrine, integumentary, skeletal,

muscle, and nervous systems.

Prerequisite(s): BIO105 General Biology with Lab or BIO110 Principles of Biology I or

accepted by CSON

4 credits Text: Principles of Anatomy and Physiology, 12th Edition Tortora / Derrickson ISBN: 0470565101

http://bcs.wiley.com/he-bcs/Books?action=index&itemId=0470084715&bcsId=4184


BIO 262 - Human Anatomy and Physiology Advanced

This course introduces the student to the basic structure and functioning of the human

body. It is the second half of a yearlong introduction to Anatomy and Physiology.

Course topics will include the organization of the body systems at the molecular,

cellular, and tissue levels and homeostatic mechanisms associated the cardiovascular,

respiratory, lymphatic, digestive, urinary and reproductive systems.

Prerequisite(s): BIO260 Human Anatomy and Physiology

4 credits Text: Principles of Anatomy and Physiology, 12th Edition Tortora / Derrickson ISBN: 0470565101

BIO 299 - Biotechnology Internship

The internship is a credit-worthy work opportunity for students to gain experience in

their major areas of study by strengthening and expanding their classroom knowledge

through practical application. Prerequisite(s): Students may intern after earning at least two-thirds of their program

credits and a 2.0 grade point average. All students must apply to intern during the

semester prior to the semester they plan to earn the internship credits.

1 credit (45 hours)



Stocked Lab Supplies Listed by Course

The following is a list of stocked items, and equipment available for each of the biology

courses. If you require additional supplies not present on the list, please contact Jill

Mitchell with your order ASAP to assure your purchases are made. In your order supply

the company name, item name, item number and quantity required.

Note: Not all additional orders are guaranteed and are based on funds available at the time.

BIO 110 Principles of Biology Storage Room C230B

Sugar

Glycerin

Dish detergent

Straws

pH paper

antacid tablets

3 beverages: i.e. soda, iced tea,

Gatorade, etc.

Milk

String

Pennies

Ethanol

Vegetable oil

Lemon juice

Ammonia

Benedicts solution

Iodine solution Buiret solution

Sudan IV solution

Starch solution

Glucose solution

Egg white

Elodea

Agar

Corn syrup

Syringes

Potato cores

Dialysis tubing

Well plates

Sodium bicarbonate

Food coloring

Yeast

Balloons

Bromothyml blue

Sucrose

Prepared mitosis slides

Magnetic chromosome kits

Electrophoresis gels, etc.

Evolutionary agents kit

BIO 115 Principles of Biology II Storage Room C230B

Under Development


BIO 210 Microbiology Storage Room C230B

Microscopes

Staining trays

Test Tube Racks

Gloves

Prepared Slides

o Letter “e” slide

o Bacterial Shapes

o Gram Stain

Glass Slides

Cover Slips

Rubber Bulb Pipettes

Toothpicks

Oil

Stains / Alcohol

o Methylene blue

o Crystal violet

o Safranin

o Nigrosin

o Safranin

o Methyl red

o Iodine

o Ethyl alcohol

o Acid fast alcohol

o Carbol fuchsin

o Methyl green

Pond Water

Sterile Swabs

Bibulous paper

Kim wipes

Petri plates

Test tubes

Sterile Cotton Swabs

Inoculating Loop

Bunsen Burners

Hot Plates

Clothespins

Wax marking pencils

Alcohol Pads

Specimen (All specimen are located in

cooler unless otherwise noted)

o Escherichia coli

o Staphlococcus aureus

o Streptococcus pyogenes

o Clostridium botulinum

o Bacillus megaterium

o Corynebacterium diphtheriae

o Micrococcus luteus

o Enterobacter aerogenes

o Serretia marcescens

o Mycobacterium smegmatis

o Saccharomyces cerevisiae

What’s in your water Kit

Agar:

o Nutrient

o Eosin Methelyne Blue

o Sabouraud agar

o MacKonkey agar

o Trypticase Soy agar

o Nutrient broth


BIO 260 Anatomy and Physiology I Storage Room C226B

Microscopes (25)

Dissecting Kits Small (12 total)

Gloves

Synthesizing Macromolecules lab Set

Dialysis Tubing

Iodine

Starch

Onion Root Tip / Ascaris Slides

Tissue Slides

Disarticulated Bones (2 boxes- green, blue)

Skeletons (4)

Muscles (see models below)

Scalpels

Mammal Spinal Cord (1 per group of 4-5)

Mammal Brain (1 per group of 4-5)

Mammal Eye (1 per group of 4-5)

Human Senses Lab Kit

Phenyl-thiocarbamide (PTC)

Thiourea

Sodium Benzoate

Note: The microscopes are rechargeable. Please make sure when you place the

microscopes back in the cabinet that they are plugged in and the timer is set for 8 hours.

BIO 262 Anatomy and Physiology II Storage Room C230B

Microscopes (25)

Gloves

Dissecting Kits

Dissecting Trays Large (10 Total)

Blood Group Identification Lab

Mammal Heart (1 per group of 4-5)

Double Injected Cats (1 per group of 4-5)

Blood Pressure Cuffs (12)

Stethoscopes (12)

Models:

See Room C226 Storage below


Lab Resources Location by Room

Prep / Storage Room C230A/B

- Microscopes

- Balances

- Hot Plates

- Stirrers

- Water Distiller

- Bunsen Burners

- Microscopes

- Autoclave

- Incubators (2)

- Fume Hood (2)

- Dishwasher

- Cooler

- Refrigerator

- Gel Electrophoresis Chambers

- Micro pipets

- Thermometers

- Glassware

- Test Tube Racks

Note: The lab equipment manuals can be found in the C230 A Prep Room in a

cabinet drawer labeled, “Equipment Manual


Prep / Storage Room C226A/B

- Beakers

- Microscopes (cordless)

- Cooler

Drawer A. Microscopy Labs

Microscopy:

Kim Wipes

Glass Slides

Cover Slips

Methylene Blue

Knife

Spoon

Tissue Example Cards

Rulers

Tissue Slides:

Fish / Onion Mitosis Set 3088164A (3)

Human Thick Skin nasco (3)

Box #1

Liver Tissue 30-8568 (4)

Box #2

Ciliated Epithelium 31-2432 (2)

Human Glandular Epithelium 31-2444 (2)

Box #3

Simple Squamous Epithelium 31-2342 (5)

Mammal Transitional Epithelium 31-2462

(3)

Box #4

Amphibian Simple Columnar Epithelium

31-2402 (4)

Box #5

Mammal Aerolar Tissue 31-2686 (3)

Box #6

Human Stratified Squamous Epithelium

31-2534 (3)

Mammal Stratified Squamous Epithelium

31-2510 (5)


Box #7

Letter “E” Wards 94 W 9111 (9)

Box #8

Letter “E” Wards 94 W 9111 (5)

Letter “E” Fisher S10227 (4)

Box #9

Ascaris Mitosis 30-8922 (5)

Onion Mitosis Root Tip 30-2396 (5)

Box #10

Ascaris Mitosis 30-8922 (6)

Onion Mitosis Root Tip 30-2396 (6)

Box #11 #12

Epithelial Tissue

A. Covering and Lining Epithelium

Mammal Simple Squamous Epithelium 31-2342

Human Simple Cuboidal Epithelium 31-2372

Human Simple Columnar Epithelium 31-2426

Human Stratified Squamous Epithelium 31-2534

Human Stratified Columnar Epithelium 31-2558

Mammal Transitional Epithelium 31-2462

Pseudostratified Ciliated Columnar Epithelia31-2498

Amphibian Ciliated Epithelium 31-2432

B. Glandular Epithelium

Human Glandular Epithelium 31-2444

Box #13, #14

Connective Tissue

A. Loose Connective Tissue

Areolar Connective Tissue 31-2686

Adipose Tissue 31-2728

Reticular Connective Tissue 31-2674

B. Dense Connective Tissue

Dense Regular Connective Tissue (see Tissue Set)

Dense Irregular Connective Tissue (see Tissue Set)

Elastic Connective Tissue 31-2764

C. Cartilage

Hyaline Cartilage 31-2898

Fibrocartilage 31-2922

Elastic Cartilage (see Tissue Slide set)

D. Bone

Bone Tissue Mammal Compact 31-2952


Bone Tissue Mammal Spongy 31-2946

E. Liquid Connective Tissue

Blood 31-3152

Box #15, #16

Skin

Skin, thick Human (Nasco) (3)

Human Pigmented Epithelium 31-4528 (1)

Human, Nonpigmented Epithelium

31-4522 (1)

Human Stratified Squamous Keratinizing

Epithelium 31-2552 (1)

Human Scalp Hair Shafts 31-4612 (1)

Human Sweat Gland Axilla 31-4595 (1)

Human Glandular Eipthelium 31-2444 (1)

Box #17 - #22

Tan Slide Boxes Include the Following Slides from Fischer Scientific

1. 9001c squamous epithelium

2. 9002F areolar connective tissue

3. 9003f hyaline cartilage

4. 9004f compact bone

5. 9005f stratified muscle

6. 9006f heart muscle

7. 9007f artery

8. 9008f vein

9. 9009f lung

10. 9010c blood smear

11. 9011f spleen

12. 9012f thyroid gland

13. 9013f thymus gland human

14. 9014f tongue

15. 9015f tooth

16. 9016f parotid gland

17. 9017f esophagus

18. 9018f stomach fundic region

19. 9019f duodenum (small intestine)

20. 9020f colon (large intestine)

21. 9021f pancreas human

22. 9022f liver

23. 9023d vermiform appendix

24. 9024f kidney

25. 9025f adrenal gland

26. 9026f ovary

27. 9027f uterus

28. 9028f placenta

29. 9029f testis, human

30. 9030f epididymis

31. 9031f cerebrum

32. 9032f cerebellum

33. 9033f spinal cord

34. 9034f sympathetic ganglion

35. 9035f skin of palm

36. 9036d scalp human hair follicles

37. 9037e scalp human hair follicles

38. 9038f retina

39. 9039 fingertip from fetus w/ nail

development

40. 9040f mammary gland


Drawer B. Osmosis / Diffusion

Osmosis Lab Materials

Thread

Dialysis Tubing

Plastic Cups

Glucose Solution

Starch Solution

Iodine

Drawer C. Biochemistry

-Macromolecules Lab Kits (5)

Drawer D. Mitosis

-Inquiries in Science Cycling Through Mitosis

-Slide Boxes #9 / #10 Ascaris Onion Root Tips Slides

Cabinet 4

Lab Materials:

Glassware

-Graduated cylinders

-Erlenmeyer flasks

-1000ml beakers

-250ml beakers

-80ml beakers

Chemicals -Iodine-Potassium Iodide Solution, 500 mL (Flinn 10027)

-Dextrose, Anhydrous, Reagent, 500 g (Flinn D0002)

-Cornstarch 500g (Flinn S0124)


Countertop

Item/ Description Company Location

a. MSDS Manual

b. Lab Addendum Handouts Binder

c. BIO 260 Materials Binder

PHCC Countertop

Eye Model (small) Eyeball only 3B Front of Shelf

Eye Model (Medium) in socket with muscle

and bone sites

3B Front of Shelf

Eye Model (Large) in socket with muscle and

bone sites

ATLAY Front of Shelf

Book Case 1


Colon Model with information Card Shelf A

Kidney Model (Small)

Cross Section With Description chart

Shelf A

Kidney Model (Med)

Cross Section on stand

Shelf A

Kidney Model (Large)

Cross Section with description booklet in

base.

Somso Modelle Shelf A

Cell Model Globe Shelf A

Liver Model (Large) Cross Section Denover Geppert

Stokie, Il

Shelf A

Brain Model in Head (Small) ELAVIL

(pharmaceutical

prop)

Shelf B


Brain Model Small (5) Shelf B

3 Heart Model (Small) Atlay Shelf C

3 Lung Cross Section Model (Small) With

Description card

Shelf C

Lung Model Pieces Shelf C

Book Case 2


Reflex Arc Model (Large, Flat) Atlay Countertop

Muscle Physiology Model Atlay Countertop

Circulatory System Model Countertop

Muscle Model

Human Body/ Head

Shelf A

Muscle Model Upper Extremities (3) Shelf B

Muscle Model Lower Limbs (3) Shelf B

Muscle Model Lower Limb


Drawer A. Human Body Organs

- Organs for human body Model

Drawer B. Human Body Organs

- Organs for human body Model

Drawer C. Human Senses

-Human Senses Kits (2)

-Vinegar

-Sugar

-Coffee

-PKU test strips

Drawer D. Misc.


Purchase Orders

Additional Lab Supplies

If you are in need of additional supplies not

found on the supply list, you will need to

complete a purchase order for the items

needed.

Here on the left you see a sample of the

purchase order form. Be sure and include

the following information when submitting

the form.

Date

Address / Phone # of company

Description

Catalog number

How many

Cost

Your name

What department

Be sure and submit your request as soon as possible to Jill Mitchell via email.

[email protected]

Note: Materials requested are purchased based on budget allowances, therefore plan

accordingly.

You may access the purchase order form here by clicking on the image above. You will be

prompted to enter your username and password to myPeak.

http://www.pennhighlands.edu/

mailto:[email protected]


Protocol

The lab and lecture rooms on campus are

used frequently for both day and evening

classes. Therefore it is of utmost

importance that the materials used

during class and lecture be returned.

ALL supplies and equipment should be

put away after use and all glassware

needs to be cleaned.

You are permitted to prep your materials

ahead of time and place the materials on a

marked cart in the prep rooms of the lab.

However following the lab sections the

materials are to be put away.

Also, it is your responsibility as an

instructor to prep your own labs. As the

instructor, you are to plan accordingly to

make sure all the materials you need are

available prior to the start of lab.

Lab specimen should be disposed of

according to the Disposal Plan located in

the Lab safety section in this manual,

page 26. You can also find copies of the

Disposal procedure in C230 prep room,

and C226 Prep room.


Lab Safety

Index

A. OSHA Laboratory Safety Guidance

B. OSHA Bloodborne Pathogens and Hazard Communication Standards

C. Pennsylvania Highlands Disposal of Biological Specimen

A. OSHA Laboratory Safety Guidance

Here are a list of helpful links to documents provided by the Occupational Safety and Health

Administration.

Laboratory Safety Guidance

OSHA Fact Sheet Chemical Hygiene Plan

OSHA Fact Sheet Laboratory Safety OSHA Laboratory Standard

OSHA Quick Facts Laboratory Safety Autoclaves / Sterilizers

OSHA Quick Facts Laboratory Safety Labeling and transferring of Chemicals

Other Helpful Links:

Material Safety Data Sheets

Center for Disease Control and BIO Safety

World Health Organization

B. OSHA Bloodborne Pathogens and Hazard Communication Standards

OSHA Fact Sheet OSHA’s Bloodborne Pathogens Standard

http://www.osha.gov/OshDoc/data_BloodborneFacts/bbfact01.pdf

http://www.osha.gov/OshDoc/data_BloodborneFacts/bbfact01.pdf

http://www.osha.gov/Publications/laboratory/OSHAfactsheet-laboratory-safety-chemical-hygiene-plan.pdf

http://www.osha.gov/Publications/laboratory/OSHAfactsheet-laboratory-safety-osha-lab-standard.pdf

https://www.osha.gov/Publications/laboratory/OSHAquickfacts-lab-safety-autoclaves-sterilizers.pdf

https://www.osha.gov/Publications/laboratory/OSHAquickfacts-lab-safety-labeling-chemical-transfer.pdf

http://www.ilpi.com/msds/index.html#Internet

http://www.cdc.gov/biosafety/

http://www.who.int/ihr/biosafety/en/

http://www.osha.gov/Publications/osha3186.pdf


C. Pennsylvania Highlands Disposal of Biological Specimen

Management and Disposal of Biological Waste

At Pennsylvania Highlands Community College

I. Introduction

The purpose of this document is to provide information, requirements, guidelines and

procedures for the handling and disposal of hazardous and non-hazardous biological

waste for all departments and units on the Pennsylvania Highlands Community

College campus. Currently there are three departments or units that generate

biowaste: Science, Healthcare Management, and Workforce Education.

“Biological Waste” or “Biowaste” means discarded biological material from teaching

and clinical laboratories and operations. This does not include household or office

trash.

“Biohazardous Waste” means any solid or liquid biological waste that is hazardous

because of its physical and/or biological nature. All waste that contains infectious

material, or because of its biological nature, may be harmful to humans, animals,

plants, or the environment is biohazardous waste. This includes waste from

infectious animals, human blood or blood products, pathological waste, and sharps.

II. Responsibility

Faculty member or other person with operational responsibility

1. Shall be responsible for ensuring students in classes generating biohazard waste

comply with the provisions of the plan.

2. Shall take appropriate steps to assure the safety of the students involved in the

handling of biowaste.

3. Will take immediate decontamination measures, primarily consisting of intensive

flushing, and other preliminary first aid procedures in the event of a spill or other

accident, while notifying the Director of Security and Safety or his employees

who will in turn notify appropriate medical and HAZMAT first responders.

Science lab manager, Health Care Management appointee, Director of

Workforce Education

1. Shall be responsible for the proper storage, handling and packaging for disposal

of biological waste generated from their respective programs. Material for


disposal shall be clearly marked in the red biohazard bags provided, and stored in

the “prep” rooms, room 224A for Healthcare Mgmt. and Workforce Ed., as well

as in room 230 for Science, pending pickup. Boxes should also be clearly marked

with the name of the originating department for billing purposes. Access to these

locked rooms shall be strictly limited to appropriate faculty, or to students under

direct supervision of those faculty members, and all access doors will be clearly

marked with signs depicting the universal biohazard symbol.

2. Shall notify the Director of Security and Safety when a sufficient number of

containers of biowaste or sharps are ready for disposal (2-3 standard boxes) so

that a pickup can be scheduled. Will also place orders for replacement containers

at this time.

3. Shall immediately report spills or other events to the Office of Security and

Safety, while ensuring that any necessary decontamination and first aid to affected

persons is immediately performed. The Office of Security will respond

immediately to the scene of the incident and will coordinate immediate

deployment of medical and HAZMAT first responders as appropriate.

Facilities Manager

1. Shall be responsible for receiving requested replacement biowaste supplies from

the disposal company at the time of a scheduled pick-up and distributing the same

to the various departments.

2. Shall be responsible for directing invoices and other paperwork resulting from a

pickup to the Director of Security and Safety, who will in turn file or distribute to

the appropriate departments as necessary.

3. Shall be responsible for transporting containers of biowaste from the “prep” room

to a holding area. This includes assuring that the containers are secure while they

await removal by the disposal company. Containers of “sharps” will be

transferred to the holding area approximately one hour prior to the anticipated

arrival of the disposal company.

4. Facilities Manager will oversee the actual loading and removal of biowaste.

Director of Security and Safety

1. Shall assume primary oversight responsibility for procedures as outlined in this

plan, as well as ensuring that they conform to applicable state and federal

regulations.

2. Shall be notified immediately of any incident occurring on PHCC property

involving biowaste spills or other related accidents, particularly those which may

result in the contamination of students, faculty, staff or others. Will coordinate

notification to appropriate medical and HAZMAT first responders, and will

proceed immediately to the accident scene to assist with decontamination and first

aid, as well as the evacuation of personnel and securing the area. Subsequent


notification of the incident will also be made to the PA Department of

Environmental Protection.

3. Following the incident will complete a PHCC Incident/Injury report, and will

convene an after-action meeting attended by all involved parties for the purpose

of identifying the cause(s) of the accident, as well as making recommendations

aimed at avoiding a reoccurrence.

4. Will ensure that all required records and notifications to regulatory agencies are

being accomplished correctly and in a timely fashion. Will maintain a file of

Department of Environmental Protection (DEP) forms # 2540, Infectious and

Chemotherapeutic Manifest, documenting pickup and eventual disposal (i.e. chain

of custody).

5. Upon notification by the referenced departments that a pickup is required, shall

contact the disposal company and make arrangements, and that information will

be relayed to the departments and to the Facilities Manager. Will also relay

orders for replacement biowaste containers as requested from the departments.

III. Segregation

1. Any waste that could produce lacerations or puncture injuries must be disposed

of as “sharps”. Sharps must be segregated from other waste. Metal sharps and

broken glass that pose a biological hazard may be comingled with each other, but

not with other waste. Sharps should not be comingled with regular laboratory

glassware which has been broken and disposed of in an appropriate and separate

container.

2. Biological waste must not be comingled with chemical waste or other laboratory

trash.

3. Biohazardous waste should be separated from other biological waste.

IV. Containers

Containers must be appropriate for the contents; not leak; be properly labeled; and

maintain their integrity if thermal or chemical treatment is used. Containers of

biohazardous materials must be kept closed.

1. Sharps—use a rigid, puncture-resistant container (heavy-walled plastic is

recommended) for storage and disposal. These are usually provided by the

disposal company.

2. Solid Biohazardous Waste—Use heavy- duty plastic “Biohazard Bags” (autoclave

bags) or containers for biohazardous waste.

3. Solid Non-hazardous Waste—Use heavy-duty plastic bag or other appropriate

container without a Biohazard symbol.

V. Storage


Biological waste may be held temporarily under refrigeration, prior to disposal, in a

safe manner that does not create aesthetic problems. Biohazardous waste should be

treated and disposed of promptly. Containers holding biohazardous material must be

clearly labeled, including the biohazard symbol.

Storage of any type of biological waste, as well as any other disposal materials, for

the various departments are as follows:

Science—the storage or prep room adjacent to Rm C230

Healthcare Management—the smaller storage room off of the large prep room

between Rms. C224 and C226.

Workforce Education—the large prep room between Rms. C224 and C226.

VI. Procedures for Treatment and Disposal

1. Microbiological Waste

a.) Should be treated (disinfected) promptly on-site by autoclaving at 15 psi and

at least 121 °C for at least 30 minutes. Autoclaving is the preferred

disinfection method. However, if autoclaving is not possible, microbiological

waste may be chemically disinfected by immersion, for not less than three

minutes, in a 1:10 (volume/volume) bleach solution.

b.) Once treated as above, the waste may be deposited in the regular trash as long

as the autoclave bag has been labeled “treated” and then placed in another bag

that is opaque—but not a biohazard bag.

2. Animal Carcasses and Body Parts

The dissection specimens that the college purchases may be disposed of

by way of the regular trash according to the manufacturer. However, it is current

policy to dispose of preserved specimens via a disposal company. Therefore,

these carcasses and body parts should be handled as follows:

a.) During the semester or session, the specimens should be double-bagged, to

prevent leakage, and stored in the refrigerator nearest the lab where the

dissection is being performed.

b.) At the end of the semester, the double-bagged specimens should be placed

into a cardboard container, supplied by the disposal company, that is lined

with a red biowaste bag. Care should be exercised to not exceed the weight

limit of the box, which is typically 50 lbs. In all cases the packaging

instructions of the disposal company should be followed (See attached).

c.) Notify the Facilities Manager when the package is ready for disposal.

3. Sharps

a.) Sharps should be placed into an approved sharps container immediately after

use. The container should be kept closed between uses.


b.) At the end of the semester or when the sharps container is full, the container

should be placed into a cardboard container, supplied by the disposal

company, that is lined with a red biowaste bag. In all cases the packaging

instructions of the disposal company should be followed (See attached).

c.) Notify the Director of Security and Safety when the package is ready for

disposal.

WEW/9-25-2

Additional Biology Resources

BIO 110 Principles of Biology

Text: Biology 8e

Campbell / Reece

ISBN: 0321543254

BIO 210 Microbiology

Text: Microbiology: An Introduction, 10e

Tortora / Funke / Case

ISBN: 0-321-55007-1

Video Tutorials via American Society of Microbiology Regional Conference 2012

Broth to Broth Transfer

Gram Stain Part 1

Gram Stain Part 2

Gram Stain Part 3

Inoculating from Solid Media

Inoculating Slant

Simple Stain

Streak Plate

Streak Plate Short

Using Micropipettors

BIO 260 Anatomy and Physiology I


http://wps.aw.com/wps/media/access/Pearson_Default/6822/6986311/login.html


Text: Principles of Anatomy and Physiology, 12th

Edition

Tortora / Derrickson

ISBN: 0470565101

BIO 262 Anatomy and Physiology II

Text: Principles of Anatomy and Physiology, 12th

Edition

Tortora / Derrickson

ISBN: 0470565101



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