0

5

10

15

20

Blank pcDNA3.0 pcDNA3.0-BTG1

0 Gy 5 Gy

Sub

-G1

perc

enta

ge

(%)

p=0.0468

0

5

10

15

20

25

30

35

Blank NC siRNA-BTG1

0Gy 5Gy

Sub

-G1

perc

enta

ge

(%) p=0.0032

0.5

1.0

1.5

2.0

2.5

3.0

0 50 100 150 200 250

Caspase-3 Colorimetric Calibration

OD 1

OD 2

OD 3

y = 0.58369 + 0.0082075x R2= 0.99976

y = 0.59068 + 0.007776x R2= 0.99966

y = 0.59592 + 0.0075328x R2= 0.9997

Abs

orb

ance

OD

(40

5nm

)

Caspase-3 (U/mL)

20

40

60

80

100

120

140

0 12 24 36 48C

asp

ase-

3 (U

/mL

)Time (h)

Activation of caspase-3 by X-ray

Supplemental data

A B

C D

E

0

10

20

30

40

NC miR-454-3p

0Gy 5Gy

Sub

-G1

pe

rcen

tage

(%

)

p=0.0139