The 13th Conference on

Fungal Genetics and

Molecular Biology

13 2013 11 20 21

1 —

2 —

11 月２０日（月） ９:30 - 受付開始（於：講演会場） 10:30 - 10:40 開会の辞 10:40 - 13:00 口頭発表（O-1～12） 13:00 - 14:20 休憩 14:20 - 15:20 特別講演 15:20 - 16:40 ポスター発表（奇数番号） 18:00 - 懇親会 11 月 21 日（火） 9:00- 受付開始（於：ポスター会場） 9:30 - 10:50 ポスター発表（偶数番号） 11:00 - 12:40 口頭発表（O-13～20） 12:40 - 14:00 昼休み 14:00 - 16:00 シンポジウム 16:10 - 16:40 総会・表彰式 16:40 - 16:50 閉会の辞

3 —

発表演題および講演時間 特別講演 11 月 20 日（水）14:20 - 15:20 糸状菌における菌体外消化システム：分解酵素の分子機構から見えてくるカビ・キノコのバイオマス資化戦略 東京大学 大学院農学生命科学研究科 五十嵐 圭日子

座長：阿部 敬悦 （東北大学） シンポジウム 11 月 21 日（木） 14:00 - 16:00 「糸状菌の多様性～種、ゲノム、機能」

[座長：S-1, 2：田中 千尋（京都大学）；S-3, 4：本山 高幸（理化学研究所）] 14:00-14:30 S-1

14:30-15:00 S-2 Dictyocatenulata alba

15:00-15:30 S-3

15:30-16:00 S-4

4 —

O-1 O-12 11 20 10:40 - 13:00 O-1, 2: ; O-3, 4, 5: ; O-6, 7, 8:

O-9, 10: ; O-11, 12: ; O-13, 14, 15: O-16, 17: ; O-18, 19, 20:

10:40 O-1

1 1 1 1 2 1 1 2

10:52 O-2 A. oryzae AoSO

11:04 O-3 Aospt3 1,2 1,2 1,2 1 2

11:16 O-4 Chaetomium globosum 1 2

, 1 , 2

11:28 O-5 RAM CoPag1

11:40 O-6 Trichoderma reesei

11:52 O-7 Trichoderma reesei 10 β-glucosidase isozyme

12:04 O-8 Cle/loxP 1)

1

12:16 O-9 Trichoderma reesei 1 2 1 3 3 4 1

1 2 3 4

12:28 O-10 3D

5 —

12:40 O-11 SSH

, , , NITE

12:52 O-12

O-13 O-20 11 21 11:00 – 12:40

11:00 O-13 A. flavus A.oryzae

11:12 O-14

, ,

11:24 O-15 1 1 1,2,3 2,3 4 1 1 ,

2 3 4 / /

11:36 O-16 Ceriporiopsis subvermispora

11:48 O-17 CreD

12:00 O-18

Kieu Pham Thi Minh, , Ba Van Vu, Quoc Bao Nguyen, ,

12:12 O-19 Aspergillus fumigatus AtrR 1 2 1 1 2

1 2

12:24 O-20

6 —

11 20 15:20 – 16:40 11 21 9:30 – 10:50 P-1 Sln1p -

1,2 3, 1, 1,2 1 , 2

, 3 Univ. of Gothenburg, Dept. of Chemistry and Molecular Biology P-2

1 , 1 , 2 1 2 P-3 11 Polyporales

CSRS David Hibett Bernard Henrrissat(CNRS) Jill Gaskel Dan Cullen (IMBT, FPL)

P-4 Cle/loxP

1) 1 P-5

1 2 3 3 1 1 2 3

P-6

P-7

P-8

1 2 1 1 1

1 2

P-9 Aspergillus oryzae

, , , , , 1, 1

, 1

P-10 L-

1 2 1 1 2 3 3 2 1 2 3

P-11 PKC

1 2 2 3 3 4, 5, 1,2 1 , 2 3 , 4 , 6

7 —

P-12 1 3 1,2 1 2 3

P13 Aspergillus oryzae HypB

P14 A. oryzae

1 1 1

P-15 Aspergillus nidulans α-1,3-

1 2 1 1,2 1 2

P-16 Mortierella alpina 1S-4 lig4

1 1 1,2 3 4 1 1 2 3 4

P-17 Aspergillus -1,3-

1 2 3 4 4 5 1, 2

(1 , 2 , 3 4 , 5 ) P-18

⁾ ⁾ ⁾

P-19 Involvement of lectin-type cargo receptors in heterologous protein secretion in Aspergillus oryzae

Dung Huy Hoang, Jun-ichi Maruyama, Katsuhiko Kitamoto (Dept. of Biotechnol., The Univ. of Tokyo) P-20 6 CoA

1 Kenneth Bruno2 1 1 1 1 Scott Baker21 1 2

P-21 A. oryzae AoRim15

P-22 Aspergillus nidulans

P-23

P-24 Aspergillus oryzae 331-25 sense RNA, 331-25 antisense RNA

8 —

P-25 A. oryzae EcdR

P-26 A. oryzae AoAtg9

P-27

P-28

( ) P-29 Aspergillus nidulans C sterigmatocystin

P-30 Aspergillus nidulans CON

a b Özlem Sarikaya Bayrama Özgür Bayrama Gerhard H. Brausa a b

P-31 Aspergillus nidulans

, P-32 HECT HulA

P-33

1 1 1 1 2 1 1 2

P-34 A. oryzae 2 acyl-CoA binding protein (AoAcb1 AoAcb2)

P-35 A. oryzae AoSO

P-36 Aspergillus oryzae

P-37 Aspergillus oryzae

, , P-38 Roles of AoLAH in regulating Woronin body position and function in Aspergillus oryzae

Pei HAN, Feng Jie JIN, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO (Dept. of Biotechnol., The Univ. of Tokyo)

9 —

P-39 DNA

P-40 A. oryzae

AoAtg26

P-41

P-42 MOA

P-43 Trichoderma reesei

1 2 1 3 3 4 1 1 2 3 4

P-44 Poly(ADP-ribose) glycohydrolase

1 1 2 2 1 1 1 2

P-45 Aspergillus oryzae ADAM

, , P-46 hydrophobin RolA

1 1 2 3 4 1,2 1

2 3 4 P-47

P-48 3D

P-49

P-50 Aureobasidium pullulans -4-

P-51 Aspergillus nidulans GfsA O- Galƒ

1 2 1 2 2 1 1 1 2

P-52

10 —

P-53 Aspergillus oryzae D-(DamA)

1 1 1 1, 5 1 1 2

3 4 4 1

1 2 3 4 5JIRCAS P-54 Aspergillus oryzae pepO

P-55 Aspergillus oryzae saccharolysin homolog

( ) P-56 Trichoderma reesei 10 β-glucosidase isozyme

P-57

P-58 hydrophobin RolA cutinase CutL1 RolA

1, 1, 1, 1, 1, 2, 3, 11 2 3

P-59

1 1, 2, 2, 2, 2, , 1 1, 1 1 , 2

P-60 Phanerochaete chrysosporium

P-61 CreA CreB

P-62 AmyR::XlnR

P-63 Trichoderma reesei

P-64 Aspergillus nidulans ManS

P-65

1 1 1 2 1 1 1 (1 2 )

11 —

P-66 Aspergillus aculeatus

P-67 Expression cluster analysis of the transcriptomes at sequential growth stages in the white-rot fungus,

Phanerochaete carnosa, grown on aspen and spruce woods. Hitoshi Suzuki (RIKEN, CSRS), Philip Wong, Chi-Yip Ho, Yunchen Gong, Kin Chan, Elisabeth Tillier and Emma Master (Univ. of Toronto)

P-68 AmyR MalR

P-69 pH PacC

1 2 1 1 1 1 1 2

P-70 xyrA larA XlnR AraR

P-71 Aspergillus aculeatus ClbR ClbR2

P-72 Molecular basis that divides ManR-dependent and ManR/McmA-dependent genes in Aspergillus

nidulans Nuo Li, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi (Grad. Sch. Bioagric. Sci., Nagoya Univ.)

P-73 Aspergillus niger PKS-NRPS

P-74 Tri6

P-75

P-76

P-77 Aospt3

1,2 1,2 1,2 1 2 P-78 Emericella variecolor

12 —

P-79 anditomin

P-80 Aspergillus fumigatus pseurotin A

P-81 Fusarium sp. FN080326 A

1 Jun-Pil JANG2 Jae-Hyuk JANG2 1 Jong Seog AHN2 1 1 CSRS 2KRIBB

P-82 Fusarium sp. RK97-94

luc1 luc4 1,2, 1, 1, 2, 2 1 2

P-83 hstD

1 2 2 1 2 (1 2 ) P-84 Aspergillus oryzae III CsyB 3-acetyl-6-alkyl-

α-pyrone 1 1 1 1 2 1

P-85 DNA

1 1 1 2 1 (1 2 ) P-86 Roxithromycin

P-87 cAMP

, P-88 Aspergillus fumigatus

, P-89 A. fumigatus

1 2 3 2 1 2

3 P-90 3 G

1, 2 1 2 1 2

13 —

P-91 ChSte20 ChCla4 1 2 1 1 1 1 2

P-92 Ras GTPase CoIra1 CoRas2 cAMP

CoMekk1-Cmk1 MAPK

P-93 MoCV1 MoCV1-B

( )

P-94 Alternaria alternata global regulator LaeA

1 1 2 1 2 3 1

1 2 3 P-95 BcAtg1

1 2 1 1 2 P-96 RAM CoPag1

P-97

1 2 3 4 1

1 2 3 4 P-98 RTS1 CoRTS1

, , ,

P-99 Enoyl-CoA hydratase

P-100 CoTEA4 CoMOD5

Special Lecture

14 —

糸状菌における菌体外消化システム：分解酵素の分子機構から見えてくる カビ・キノコのバイオマス資化戦略

CBHCDH CBH

-1,4

CDH

-Fe3+

1. 1-5

Phanerochaete chrysosporium CDH

pH

2. 6-9

Trichoderma reesei 7CBH TrCel7A

1 "m 1,000

15 —

E212Q TrpW40A

-7 -1 +1 +2

3.

CDH TrCel7A

1. Igarashi, K. et al. Fung. Genet. Biol. (1997) 2. Igarashi, K. et al. Eur. J. Biochem. (1998) 3. Igarashi, K. et al. J. Biol. Chem. (1999) 4. Igarashi, K. et al. Biochem. J. (2002) 5. Igarashi, K. et al. FEBS J. (2005) 6. Igarashi, K. et al. FEBS J. (2006) 7. Igarashi, K. et al. FEBS J. (2007) 8. Igarashi, K. et al. J. Biol. Chem. (2009) 9. Igarashi, K., Uchihashi, T. et al. Science (2011) Extracellular digesting-system in filamentous fungi: Fungal strategy for the biomass utilization “visualized” from molecular mechanisms of degrading enzymes Kiyohiko Igarashi (Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo)

Symposium

16 —

S-1

15010 30 150

1 5

DNA 5001000

1000

3000

17 —

20114 1 1000 3500

2010

18 —

S-2 Dictyocatenulata alba

(RIKEN BRC-JCM)

Dictyocatenulata alba Finley & E.F. Morris

; ; 1 “1 ” “ (hyphomycete)”[1,2]

Morris and Finley[3] (anamorphic fungus) [4]

[2]

[1,4] [1,5]

D. alba

[5]

1) D. alba 11 5 2) (JCM 5358, 5359, 5360, 8401, 12918) DNA

PCR [nuSSU, nuLSU (D1/D2), ITS]

3) nuSSU

RT-PCR

1) D. alba 5

Lecanoromycetes Ostropomycetidae

2) nuLSU ITS (JCM 5359, 5360) (JCM 8401, 12918) (JCM 5358)

3) nuSSU a. I

19 —

b. 5 D. alba 2 5

c. d. 5 3 nuLSU ITS

e. D. alba

4) 5

nuLSU ITS nuSSU –; JCM 5358, 5359, 8401 D. alba 3 (cryptic species)

species complex 5) – ; PDA, 20C 12

D. alba (lichenized fungus)

; D. alba

6) 3 D. alba Gyalecta (Ostropales, Ostropomycetidae)

(KPM-NC0017988) D. albanuLSU ITS

- - 7) (Chocorua, NH, USA) D. alba

/ D. alba

–

20 —

[1] Seifert et al. 1987. Mycologia 79: 459–462. [2] Seifert et al. 2011. The genera of hyphomycetes. CBS biodiversity series, vol. 9 (ISBN

978-90-70351-85-4; http://www.generaofhyphomycetes.org/). [3] Morris and Finley. 1967. Am Midl Nat 77: 200–204. [4] Diederich et al. 2008. Sauteria 15: 205–214. [5] An et al. 2012. Fung Biol 116: 1134–1145.

21 —

S-3

4000 3000

4000 3500 3000

1986 Rhizopus Absidia

2008 Aspergillus oryzae

A. oryzae

A. oryzae1990 1876

Black2005 1000Aspergillus oryzae

A. oryzae

A. oryzaeA. oryzae

2009NRRL

Northern Regional Research Laboratories

A.oryzae

NRRL 467 1920 K. Oshima TL Ao 5c Ueda, Osaka = Thom 290-4429-Ao 5c

NRRL 466 1920 K. Oshima TL Ao 5b Ueda, Osaka = Thom 290-4429-Ao 5b

NRRL 460 1920 K. Oshima TL Ao 1 = Thom 290-4429-Ao 1

NRRL 1911 1943 S.A. Waksman TL No.35

NRRL 461 1920 K. Oshima TL Ao N

22 —

= Thom 290-4429-AoN

NRRL 462 1920 K. Oshima TL Ao P = Thom 290-4429-Ao P

NRRL 455 1920 K. Oshima TL= Thom 290-4429-A

NRRL 456 1920 K. Oshima TL = Thom 290-4429-Ao

NRRL 457 1920 K. Oshima TL = Thom 290-4429-Ao 6

NRRL 458 1920 K. Oshima TL = Thom 290-4429-Ao-Old

NRRL 459 1920 K. Oshima TL= Thom 290-4429-Ao K

NRRL 463 1920 K. Oshima TL = Thom 290-4429-Ao 2a

NRRL 464 1920 K. Oshima TL = Thom 290-4429-Ao 4a

NRRL 1919 1943 S.A. Waksman TL No.42

1 TL Aspergillus oryzae Wicklow 2002

Elander 2002 SIM News1978

1948”Cephalosporium acremonium”

Cephalosporium

acremonium CephalosporiumAcremonium Acremonium strictum

A. chrysogenum A. strctum Gams, 1971A. strictum A. strictum

Sarocladium strictum Summerbell et al., 2011

2013 1 1 1 1Acremonium

Rossman et al., 2013

Tolypocladium

inflatum Penicillium citrinum Aspergillus terreus Coleophoma sp. 1970 1990

1993

23 —

iPS

Bills (2013)

http://www.kitasato.ac.jp/ NP Depo http://www.npd.riken.jp/npd/ja/

http://www.meti.go.jp/policy/tech_ promotion/kenkyuu/saishin/42.pdf

2012

http://www.opbio.com/http://www.hyphagenesis.co.jp

HGI2006 TAMA 10

StrMyco 22,000 ExMyco 41,000 PowKinoco 1,200

TAMA

Hypocreales

15,000 7001 10% 0.5%

600TAMA

NBRC 252 587

HGIExMyco

24 —

MS ACCESS

ExMycoFusarium

incarnatum C NA 255Nagahashi et al., 2009

Pochonia suchlasporia pochonicine NUsuki et al., 2009

Hypomyces pseudocorticiicolaF 2928-1 F 2928-2 Kanai et al.,

2005 Mariannaea M. camptosporamariannin A B Fukuda et

al., 2011 Simplicillium lanosoniveum preussin2012

20094

ExMyco 38,8002 NH1

in silicoNH4

25http://www.chusho.meti.go.jp/keiei/sapoin/2013/0731globalkoubo.pdf

2010 CBD COP10

3CBD

1992 JBACBD

Saccharomyces cerevisiae Wang et al., 2012

25 —

CBS-KNAW Fungal

Biodiversity Centre, Centraalbureau voor Schimmelcultures, CBSCBS

2012 Stagonospora paludosa 300

http://connect.barcodeoflife.net/forum/topics/at-the-cbs-we-are-looking-for-the-type-of-stagonospora-to-barcode

TAMA2012 231

4TAMA

Bills GF, Gloer JB, An Z. 2013. Coprophilous fungi: antibiotic discovery and functions in an

underexplored arena of microbial defensive mutualism. Current Opinion in Microbiology. 16: 1-17.

Black JG 2005 (2007) 1. In 2

1-27pp. Elander RP. 2002. University of Wisconsin contributions to the early development of Penicillin

and cephalosporin antibiotics. SIM News 52: 270-278. Fukuda T, Sudoh Y, Tsuchiya Y, Okuda T, Fujimori F, Igarashi Y. 2011. Marianins A and B,

prenylated phenylpropanoids from Mariannaea camptospora. Journal of Natural Products. 74: 1327–1330

Gams W. 1971. Cephalosporium-artige Schimmelpilze (Hyphomycetes). G. Fischer Verlag, Stuttgart. 262pp.

Kanai Y, Tatsumi Y, Tokiwa T, Watanabe Y, Fujimaki T, Ishiyama D, Okuda T. 2005. F2928-1 and -2, new antifungal antibiotics from Cladobotryum sp. Journal of Antibiotics 58: 507-513.

Koizumi T. 2004. Sake brewery as traditional and advanced technologies. In Proceedings of The 10th International Congress for Culture Collections (ICCC-10) Open Seminar “Traditional Koji mold entering a modern gate” edited by Okuda T & Bennet J. 28-33pp.

Nagahashi Y, Ito T, Masubuchi M, Yamaguchi Y, Sakamoto H, Sudoh M, Kawasaki K, Tsukuda T, Katoh H, Okuda T, Aoki M. 2009. NA255: anti-hepatitis C virus agent produced by Fusarium incarnatum and fermentation of NA808, a derivative of NA255. Proceedings of SIM Annual Meeting and Exhibition. Westin Hrbour Castle, Toronto, ON Canada, July 26-30. P96.

Rossman AY, Seifert KA, Samuels GJ, Minnis AM, Schroers HJ, Lombard L, Crous PW, Poldmaa K, Cannon PF, Summerbell RC, Geiser DM, Zhuang WY, Hirooka Y, Herrera C, Salgado-Salazar C, Chaverri P. 2013. Genera in Bionectriaceae, Hypocreaceae, and Nectriaceae (Hypocreales) proposed for acceptance or rejection. IMA Fungus 4: 41-51.

Summerbell RC, Gueidan G, Schroers HJ, de Hoog GS, Starink M, Rosete YA, Guarro J, Scott JA. 2011. Acremonium phylogenetic overview and revision of Gliomastix, Sarocladium,

26 —

and Trichothecium. Studies in Mycology 68: 139-162. Usuki H, Toyo-oka M, Kanzaki H, Okuda T, Nitoda T. 2009. Pochonicine, a polyhydroxylated

pyrrolizidine alkaloid from fungus Pochonia suchlasporia var. suchlasporia TAMA 87 as a potent beta-N-acetylglucosaminidase inhibitor. Bioorganic & Medicinal Chemistry 17: 7248-7253.

Wang QM, Liu WQ, Lit G, Wang SA, Bai FY. 2012. Surprisingly diverged poplulations of Saccharomyces cerevisiae in natural environments remote from human activity. Molecular Ecology 22: 5404-5417.

Wicklow DT, McAlpin CE, Peterson SW. 2002. Common genotypes (RFLP) within a diverse collection of yellow-green aspergilla used to produce traditional oriental fermented foods. Mycoscience. 43: 298-297.

2008 1. In 73-93pp.

2009 46: 10-18. 1978 237pp.

1986 In 487-496pp.

2011 Simplicillium lanosoniveum (

) 3A04p03 2012

2012 5961 2012 10 11

Fungal Bank and its Industrial Application Toru Okuda (Mycology & Metabolic Diversity Research Center, Tamagawa University Research Institute)

27 —

S-4

PCR

NRP

kb

28 —

1 2

3,4 References 1: Ishidoh K et al 2013. Curr. Genet in press 2: Shimizu T et al 2005. Appl Environ Microbiol 71:3453-3457. 3: Sakai K et al 2009. Biotechnol Lett. 31:1911-6 4: Sakai K et al 2012 Appl Microbiol Biotechnol. 93:2011-22.

Oral Session

29 —

O-1 (P-33)

1 1 1 1 2 1 1 2

Virocontrol

Propidium iodideFUN-1

RNAseqresponse to stimulus, transport

Cytological and transcriptional analyses of heterogenic incompatibility in Rosellinia nacatrix. 1Takahiro Uwamori, 1Kanako Ikeda, 1Chiaki Kida, 1Hitoshi Nakayasiki, 2Satoko Kanematsu, 1Kenichi Ikeda

(1Grad. Sch. Agric. Sci, Kobe Univ. 2Nat. Inst. Fruit Tree Sci. NARO)

O-2 (P-35) A. oryzae AoSO

Aspergillus oryzae

A. oryzaeAoSOAoSO

AoSO 3 N 1-555C 1147-1195

AoSO C N

AoSOAoSO 2

AO090005001564 AosoAO090005001564 AoBni1

AoSO AO090005001564

Investigation of AoSO function in cell repair after hyphal wounding in Aspergillus oryzae Junpei KAWABATA, Kei SAEKI, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO (Dept. of Biotechnol., The Univ. of Tokyo)

30 —

O-3 (P-77) Aospt3

1,2 1,2 1,2 1 2

Aospt3secondary metabolites SMs

SMs Aospt3 SMs SMs ΔAospt3

(kojA kojR kojT) 100Aospt3

SMs laeAAospt3 laeA ΔAospt3 laeA

ΔAospt3ΔlaeAΔlaeA

Aospt3 laeA ΔlaeAspt3 ΔAospt3 laeA

Aospt3 laeA

Functional analysis of Aospt3 secondary metabolic regulator

Masato Hirose , Moriyuki Kawauchi, Kazuhiro Iwashita (Hiroshima Univ, NRIB)

O-4 Chaetomium globosum

1 2 , 1 , 2

Chaetomium globosum.

[Nakazawa et al. (2013) J. Am. Chem. Soc., in press]CGKW14; ligD pyrG

Velvet A CgveA chaetoviridin cochliodinol

aureonitol, chaetoglocin SPT10CgsptJ mollipilin coactatin

aureonitolchaetoviridin

ChIPaureonitol/mollipilin chaetoglocin/coactatin

H3K9 trimethylation CgveAcochliodinol chaetviridin

CgveA

Alternation of biosynthetic regulation and its effect on epigenetic states in Chaetomium globosum.

Takehito Nakazawa, Kan’ichiro Ishiuchi, Yasutaka Gotanda, Hiroshi Noguchi, Kenji Watanabe.

(School of Pharmaceutical Sciences, University of Shizuoka)

31 —

O-5 (P-96) RAM CoPag1

CoKEL2cokel2Δ

CoKEL2 6

RAM (regulation of Ace2p activity and cellular morphogenesis)Pag1 (Tao3) CoPAG1 copag1Δcokel2Δ

CoPAG1CoPag1::RFP

CoPag1::RFP

Colletotrichum orbiculare CoPag1, a component of RAM network in Saccharomyces cerevisiae, is involved in

appressorium development triggered by plant-derived signals

Sayo Kodama, Ayumu Sakaguchi, Yasuyuki Kubo

(Graduate School of Life and Environmental Sciences, Kyoto Prefectural Univ.)

O-6 (P-63) Trichoderma reesei

Trichoderma reesei

PC-3-7 MFSTRP3405 PC-3-7

TRP3405PC 3405 PC-3-7

PC 3405PC 3405 PC-3-7

Influence of gene destruction of cellulose specific transporter in Trichoderma reesei

Hiroki Taniguchi, Hideyuki Kusaka, Tkanori Furukawa, Yosuke Shida, Wataru Ogasawara

(Nagaoka Univ. of Tech)

32 —

O-7 (P-56) Trichoderma reesei 10 β-glucosidase isozyme

Trichoderma reesei β-glucosidase (BGL) BGL

10 BGL (Cel1A Cel1B Cel3A Cel3B Cel3C Cel3D Cel3E Cel3F Cel3G Cel3H)

GH1 Cel1A Cel1B GH3 BGL

BGL p-Nitrophenyl-β-D-glucoside (pNPG1) (G2) Cel3H BGL BGL

pNPG1 G2 Cel3A Cel3BCel3E Cel3C Cel3D Cel3F Cel3G

Cel1B pNPG1 G2 Cel1A G2 BGL 10% G2 pNPG1

BGL G2 BGL (Cel1 Cel3ACel3B Cel3E)

Cel1A Cel3A5.4 mg/ml 2.8 mg/ml Cel3A

Cel1A Cel1A

Enzymatic properties of β-glucosidase isozymes from Trichoderma reesei

Boyang Guo, Kouichi Nozaki, Masahiro Mizuno, Yoshihiko Amano

(Dept. of Biosci. & Textile Technol., Interdisciplinary Graduate School of Sci. and Technol., Shinshu Univ.)

O-8 (P-4) Cle/loxP

1) 1

, ,

, lox Cre lox Cre/loxP, 2012 , Cre

, Cre,

adeA xynG2 Cre lox

, Cre, Cre ,

adeA , Cre adeA Cre , ligD

xynG2 , , Cre Penicillium chrysogenum (xylP)

, adeACre , adeA Cre

,

Development of a self-excising Cre/loxP marker recycling system in Aspergillus oryzae

Silai Zhang, Akihiko Ban, Naoki Ebara, Osamu Mizutani1), Mizuki Tanaka,TakahiroShintani, KatsuyaGomi

(Grad.Sch,Agric.Sci.,Tohoku Univ., NRIB1))

33 —

O-9 (P-43) Trichoderma reesei 1 2 1 3 3 4 1

1 2 3 4

Trichoderma reesei

FT-IR

T. reesei6

GPIT. reesei GPI

Exploration of protein related to cell surface fibrous material synthesis of Trichoderma reesei

Shingo Tahara1, Mikiko Nitta2, Yosuke Shida1, Yoshiki Horikawa3, Junji Sugiyama3, Masako Osumi4, Wataru

Ogasawara1

(1Nagaoka Univ. of Technol., 2JST, 3RISH, Kyoto Univ., 4IIRS)

O-10 (P-48) 3D

Coleophoma empetri F-11899FR901379 A AAC

Micafungin AAC

AAC

AAAC A

CAPCR Pseudomonas sp. SY-77 CA

AAAC

Substrate switching of antibiotics acylase by 3D modeling Kazuki Nakayama, Masato Yamada, Hiromi Nishida, Yasuhiro Isogai (Toyama Pref. Univ., Dept. Biotech. )

34 —

O-11 SSH

, , , NITE

SSHYeast

ext., Sucrose, Soluble starch SSH 300Illumina MiSeq

17 SSH Blast

NEDO

Investigation of fungal SSH-libraries for biomass decomposition

Sonyon Ann, Akihiro Oguchi, Natsuko Ichikawa, Tomohiro Sekigawa, Kei Kamino

(NITE)

O-12

Production of useful materials from fungi with blue light stimulation

Masanobu Kojima1, Ryuhei Miura1, Satoshi Mihara2, Masaki Ichimura2

(1Grad. Sch. Agri., Shinshu Univ.; 2JA Nakanoshi)

35 —

O-13 A. flavus A.oryzae

Aspergillus flavusA. oryzae A. flavus ,

,A. flavus Aspergillus ,Circumduti ,Flavi A.oryzae

2,

ATCC,ARS Culture collection,NRRL A. flavus 31 2 NBRC NRRLITS ,ISSR DNAchip

ITS

,6 A.sojaeA. oryzae 13 ISSR1 (GTG)5 ISSR2(GACA)4

ISSR , 3A. oryzae , A. flavus

, ISSR A. flavus DNAchip

A. oryzae 13 A. flavus Gene springA. flavus A. oryzae

Genome wide phylogenetic analysis of Aspergillus flavus and A. oryzae

Tamy Ohota, Ken Oda, Kazuhiro Iwashita, Nami Goto (NRIB)

O-14

, ,

NO

NO

Aspergillus nidulans NO

ntpA ntpA cysteine-rich 23 aa NO ntpA

NO NO

NO-inducible

nitrosothionein (iNT) iNT thioredoxin thioredoxin reductase

iNT iNT NO iNT

iNT NO

Discovery of an NO-resistant peptide nitrosothionein from Aspergillus nidulans.

Shengmin Zhou, Toshiaki Narukami, Shunsuke Masuo, Naoki Takaya

(Graduate School of Life and Environmental Sciences, Univ. of Tsukuba)

36 —

O-15

1 1 1,2,3 2,3 4 1 1 , 2

3 4 / /

660 nm 528 nm 467 nm 375 nmLED

JPS56131319(A)-1981-10-14 2

2

WC1 WC2 Gf.WC1 Gf.WC2

Gf.BMR1Bipolaris oryzae BMR1

Gf.BMR1Gf.BMR1

Gf.BMR1

Grifola frondosa distinguishes between blue light and near-ultraviolet light.

Atsushi Kurahashi1, Shimoda Takafumi1, Masayuki Sato1,2,3, Fujimori Fumihiro2,3, Jyuniji Hirama2, Kozo Nishibori1

(1Yukiguni Maitake, 2 Hyphagenesis, 3Tokyo Kasei Univ. 4Kanazawa Inst. Tech. EOE)

O-16 Aspergillus fumigatus

Ceriporiopsis subvermispora

Ceriporiopsis subvermispora

Phanerochaete chrysosporium C.

subvermispora C. subvermispora

ceriporic acid

Suppression Subtractive Hybridization

Vanillin-induced cellular response in the selective white rot fungus, Ceriporiopsis subvermispora

Takahito Watanabe, Kenichiro Mori, Ayako Kido, Takahiro Hasegawa, Hiroshi Nishimura, Yoichi Honda, Takashi

Watanabe (RISH, Kyoto Univ.)

37 —

O-17 CreD

CreD , , ,

CreB ( , 2012), CreD( , 2012) , CreD

CreD , CreD

creB creD creB , CreDCreD Rod1 , Snf1

, CreD 2 Snf1, , 2

creB CreD, , CreD

, CreD HECT HulA, CreD HulA

Regulation of glucose repression through dephosphorylation of arrestin-like protein CreD in Aspergillus

oryzae.

Mizuki Tanaka, Tetsuya Hiramoto, Takahiro Shintani, Katsuya Gomi

(Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ.)

O-18

Kieu Pham Thi Minh, , Ba Van Vu, Quoc Bao Nguyen, ,

7HMT HMT

MoHMT4SET1

MoHMT4 H3K4H3K4me2 ChIP-seq

H3K4me2 RNA-seqMoHMT4

MoHMT4 MoHMT4

Histone lysine methyltransferases are involved in various aspects of pathogenesis in Magnaporthe oryzae.

Kieu Pham Thi Minh, Yoshihiro Inoue, Ba Van Vu, Quoc Bao Nguyen, Ken-ichi Ikeda, Hitoshi Nakayashiki.

(Fac. of Agri., Kobe Univ.)

38 —

O-19 Aspergillus fumigatus AtrR

1 2 1 1 2 1 2

Aspergillus fumigatus

A. fumigatus AtrR

bHLH SrbA(cyp51A, erg3,

erg24A, erg25A) atrR srbA atrR srbA

ABC AbcC/CdrB

srbA abcC/cdrB atrRabcC/cdrB AtrR

ABC

AtrR plays a crucial role in azole-resistance in Aspergillus fumigatus

Daisuke Hagiwara1 Ayumi Ohba2 Kiminori Shimizu1 Susumu Kawamoto1 Katsuya Gomi2

1MMRC, Chiba Univ. 2Grad. Sch. of Agric. Sci., Tohoku Univ.

O-20

Colletotrichum orbiculare

3 NIS1 CoDN3 CoMC69

CoDN3

SEC4SEC4 ER-Golgi SEC22

ER-Golgi

Effector Delivery toward Ring-Shaped Interface Developed between Colletotrichum orbiculare and Its Host

Cucumber

Hiroki Irieda, Hitomi Maeda and Yoshitaka Takano

(Grad. School of Agriculture, Kyoto Univ.)

Poster Session

39 —

P-1 Sln1p -

1,2 3, 1, 1,2 1 , 2 , 3 Univ.

of Gothenburg, Dept. of Chemistry and Molecular Biology

AtETR1 (HK)HK

(EBD) HK Sln1p HK HKTcsB HKD HK HK

AtETR1 EBD Sln1p Aspergillus nidulans TcsB HKD HK

Sln1p ScSln1pAtETR1(EBD)-ScSln1p(HKD) AtETR1(EBD)-AnTcsB(HKD) ScSln1p HK

HK HOG

Functional complementation and ethylene response of plant-fungal fusion histidine kinase in a

temperature-sensitive sln1 yeast mutant.

Mayumi Nakayama1,2, Kentaro Furukawa3, Akira Yoshimi1, Keietsu Abe1,2

(1NICHe., Tohoku Univ., 2Grad. Sch. Argric. Sci., Tohoku, Univ., 3 Univ. of Gothenburg, Dept. of Chemistry and

Molecular Biology)

P-2

1 , 1 , 2 1 2

0-24 DNA

PCR

DGGE

DNA

24 3141

Effects of aging treatment on bacterial community in bamboo sawdust 1Yuji Nakada, 1Makoto Yoshida, 2Koji Takabatake

(1Tokyo Univ. of Agric. and Technol., 2Toyama Prefectural Agric, Forestry and Fisheries Research Center, Forestry

Research Institute)

40 —

P-3 11 Polyporales

CSRS David Hibett Bernard

Henrrissat(CNRS) Jill Gaskel Dan Cullen (IMBT, FPL)

Polyporales

11 Polyporales

3 Bjerkandera adusta Ganoderma sp. Phlebia brevispora 11 Polypore CAZy

75 -

±

±

Genome-wide analysis of polysaccharides degrading enzymes in eleven white- and brown-rot Polyporales

Chiaki Hori (CSRS, RIKEN), Kiyohiko Igarashi, Masahiro Samejima (Agri., Univ. of Tokyo), David Hibbett (Clark

Univ), Bernard Henrissat (CNRS), Jill Gaskel and Dan Cullen (USDA-FPL) P-4 (O-8)

Cle/loxP 1) 1

,

, , lox Cre lox Cre/loxP

, 2012 , Cre , Cre

, adeA xynG2 Cre lox

, Cre, Cre ,

adeA , Cre adeA Cre , ligD

xynG2 , , Cre Penicillium chrysogenum (xylP)

, adeACre , adeA Cre

,

Development of a self-excising Cre/loxP marker recycling system in Aspergillus oryzae

Silai Zhang, Akihiko Ban, Naoki Ebara, Osamu Mizutani1), Mizuki Tanaka,TakahiroShintani, KatsuyaGomi

(Grad.Sch,Agric.Sci.,Tohoku Univ., NRIB1))

41 —

P-5

1 2 3 3 1 1 2

3

Monitoring filamentous fungi existing in the cultural properties from infinitesimal quantity samples

Tomoko Wada1, Kiyohiko Igarashi2, Yuko Fujiwara3, Yoshihisa Fujii3, Ken Okada1

(1Cent. for Conservation and Restoration Techniques, Nat. Res. Ins. for Cultural Properties, Tokyo, 2Grad. School of

Agricultural and Life Sciences, Univ. of Tokyo, 3Grad. School of Agriculture, Kyoto Univ.)

P-6

1, 2, 3, , 1, 2, 2, 4, 5, 5, 3, 1, 6 1 , 2 , 3 , 4 ,5

, 6

hydrophobin(-RolA) Aspergillus fumigatus hydrophobin ( )

RolA

(Fe3O4) ( 200 nm) RolA

in vivo RolA RolA

pH

Development of imaging nano-particles coated with immune-response free (stealth) fungal materials

Daiki Sato1, Kana Matumura2, Shintaro Takahashi3, Kimihide Muragaki1, Ishi Keiko2, Kazuyoshi Kawakami2,

Takanari Togashi4, Seiichi Takami5, Masafumi Ajiri5, Manabu Fukumoto3, Keietsu Abe1, 6

(1Grad Sch Agric Sci Tohoku Univ, 2Grad Sch Med Tohoku Univ, 3IDAC Tohoku Univ, 4Sci Yamagata

Univ.5IMRAM Tohoku Univ, 6NICHe Tohoku Univ)

42 —

P-7

1 1

Production of materials with anticancer activity from oyster mushroom mycelia by blue light stimulation

Ryuhei Miura1, Kazuya Toda1, Hiroshi Fujii1, Masanobu Kojima1, Satoshi Mihara2, Masaki Ichimura2

(1Grad. Sch. Agri., Shinshu Univ.; 2JA Nakanoshi)

P-8

1 2 1 1 1 1 2

Lyophyllum shimejiAgrobcterium tumefaciens pPZP-HYG2

EHA104PCR 20

A. tumefaciens 3 6

TAIL-PCR

Agrobacterium tumefaciens Mediated Transformation of Lyophyllum shimeji

Mayumi Yamamoto1, Kosuke Izumitsu2, Yuki Kitade1, Kanako Hatoh1, Chihiro Tanaka1

(1Grad. Sch. of Agri., Kyoto Univ., 2Sch. of Environmental Science, Univ. of Shiga Pref. )

43 —

P-9 Aspergillus oryzae

, , , , , 1, 1

, 1 [ ] Aspergillus

(PLoS ONE(2013) 8, e62512)

[ ] A. oryzae ∆Aoatg8 amyB

12 ∆Aoatg8 1 2

EGFP

Improved production of a heterologous protein in Aspergillus oryzae by autophagy deficiency.

Hiroshi Teramoto, Kiyomi Kagami, Kaihei Kojima, Hiroaki Udagawa, Shinobu Takagi, Jun-ichi Maruyama1,

Katsuhiko Kitamoto 1

(Novozymes Japan Ltd, 1 Department of Biotechnology, The University of Tokyo)

P-10 L-

1 2 1 1 2 3 3 2 1

2 3

Aspergillus oryzae L-

A. oryzae RIB40

lactate dehydrogenase (LDH)LDH A. oryzae LDHΔ871 100 g/L 50 g/L

A. oryzae LDHΔ871 100 g/L 30 g/L 99.9% L

L-

L-lactic acid production from starch by simultaneous saccharification and fermentation in Aspergillus oryzae

Satoshi Wakai1, Toshihide Yoshie2, Nanami Asai1, Ryosuke Yamada1, Chiaki Ogino2, Hiroko Tsutsumi3, Yoji Hata3,

Akihiko Kondo2

(1Org. Adv. Sci. Tech., Kobe Univ., 2Dept. Chem. Sci. Eng., Grad. Sch., Kobe Univ., 3Res. Inst., Gekkeikan)

44 —

P-11 PKC 1 2 2 3 3 4, 5, 1,2 1

, 2 3 , 4 , 6

CWISC PKC

Aspergillus nidulans PKC AnPkcA PKC MgPkc1PKC

MgPkc1 MgPkc1

MgPkc1 Z-705 Z-705 Z-705

PKC1 PKC PKC Z-705

Z-705 PKCPKC PKC1

Construction of yeast screening system for novel chemical agents that inhibit PKC of filamentous fungi.

Fumio Shoji1, Mayumi Nakayama2, Akira Yoshimi2, Tomonori Fujioka3, Kiyoshi Kawai3, Hiroyuki Horiuchi4,

Hideaki Umeyama5, Keietsu Abe1,2 (1Grad. Sch. Argric. Sci. & 2NICHe. Tohoku Univ., 3Kumiai Chemical Industry

Co., Ltd., 4 Grad. Sch. Agric. Sci. Tokyo Univ., 5Facul. Sci. Engin. Chuo Univ.)

P-12

1 3 1,2 1 2 3

Aspergillus oryzae

(CFGD) CFGD(Comprehensive Aspergillus oryzae genome database: CAoGD)

http://nribf21.nrib.go.jp/CFGD/ RIB40 9 27

Roche 454 GS FLX titanium 37 RIB RIB40

AspGD A. nidulans FGSC A4 A. niger CBS513.88

Development and publication of Comprehensive Aspergillus oryzae Genome Database (CAoGD) Ken Oda1, Yasuo Uemura3, , Kazuhiro Iwashita1,2 (1NRIB, 2Hiroshima Univ., 3Genaris Inc.)

45 —

P-13 Aspergillus oryzae HypB

Hydrophobin

Hydrophobin Hydrophobin

Aspergillus oryzae HydrophobinhypA~C hypB

HypBhypB egfp A. oryzae

HypB-EGFP Proteinase KEGFP HypB

HypB

Construction of cell surface display systems using hydrophobin (HypB) with functional peptides in

Aspergillus oryzae

Shintaro Ohashi, Keisuke Domae, Satoshi Nakama, Asuka Kase, Harushi Nakajima

(Dept. of Agricultural Chemistry, Univ. of Meiji)

P-14

A. oryzae 1 1

1

1)

Aspergillus oryzae

A. oryzae cDNA A. oryzae tef1

cDNA amyBP-glaA142 HA His6

AmyB Kex2A. oryzae 10 NSlD-ΔP10 2) 5 DPY 4

Ni-NTASaccharomyces cerevisiae

Pho36p AO090003000602, AO090026000756 A. oryzae

1) Narasimhan et al. (2005) Mol. Cell 17, 171-180. 2) Yoon et al. (2011) Appl. Microbiol. Biotechnol. 89, 747-759.

Production and purification of plant osmotins in Aspergillus oryzae

Hiromasa ITO, Jun-ichi MARUYAMA, Koji NAGATA1, Masaru TANOKURA1, Katsuhiko KITAMOTO

(Dept. of Biotechnol., The Univ. of Tokyo; 1Dept. of Appl. Biol. Chem., The Univ. of Tokyo)

46 —

P-15 Aspergillus nidulans α-1,3-

1 2 1 1,2 1 2

AG AG

AGAG

AG AG

AGAG

Morphologic properties and penicillin production of Aspergillus nidulans α-1,3-glucan deficient mutants

under liquid culture conditions. Masahiro Hitosugi1, Akira Yoshimi2, Azusa Inaba1, Keietsu Abe1,2(1Grad. Sch. Sci.,& Tohoku,Univ. 2NICHe, Tohoku Univ.)

P-16 Mortierella alpina 1S-4 lig4

1 1 1,2 3 4 1 1 2

3 4

(PUFA)

Mortierella alpina 1S-4 PUFA PUFAPUFA

DNA LIG4lig4 lig4

PCR lig4lig4 lig4

93 3 lig4 3%lig4 4 1

25% lig4

A lig4 gene-disrupted strain from oleaginous fungus Mortierella alpina 1S-4

Hiroshi Kikukawa1, Eiji Sakuradani1, Akinori Ando1,2, Misa Ochiai3, Sakayu Shimizu4, Jun Ogawa1 (1Div. Appl.

Life Sci., Grad. Sch. Agric., Kyoto Univ., 2Res. Uni. Physiol. Chem., Kyoto Univ., 3Suntory Global Innovation

Center Ltd., 4Fac. Bioenviron. Sci., Kyoto Gakuen Univ.)

47 —

P-17 Aspergillus -1,3-

1 2 3 4 4 5 1, 2 (1 , 2

, 3 4 , 5 )

Aspergillus A. oryzae A. nidulans -1,3-

AG A. nidulans AG AGCongo Red - Lysing Enzymes

AGAG

Histoplasma capslatum Magnaporthe grisea AG

AG AG AG

AGAG

AG 2 25 AGAG AG

AG

Production of alpha-1,3-glucan oligosacchalides from cell wall of Aspergilli and its functional analysis

Akira Yoshimi1, Azusa Inaba2, Shigekazu Yano3, Hiroaki Sasaki4, Tomoo Goto4, Kazuyoshi Kawakami5, Keietsu

Abe1, 2 (1NICHe, 2Grad. Sch. Agric. Sci., 5Sch. of Med. Tohoku Univ., 3Grad. Sch. Sci. & Eng. Yamagata Univ., 4Gifu Shellac MFG) P-18

⁾ ⁾ ⁾

Phoma betae DNA polymeraseAspergillus oryzae ⁾

TP$

GFP RFP

2 Cytochrome P450

TP

1) Fujii et al., Biosci. Biotechnol. Biochem., 75, 1813-1817 (2011)

Subcellular localization of aphidicolin biosynthetic enzymes expressed in the heterologous host, Aspergillus

oryzae.

Akihiko Ban, Mizuki Tanaka, Ryuya Fujii¹⁾, Atsushi Minami¹⁾, Hideaki Oikawa¹⁾, Takahiro Shintani, Katsuya Gomi ( Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ., ¹Hokkaido Univ.)

48 —

P-19 Involvement of lectin-type cargo receptors in heterologous protein secretion in Aspergillus oryzae Dung Huy Hoang, Jun-ichi Maruyama, Katsuhiko Kitamoto (Dept. of Biotechnol., The Univ. of Tokyo)

Aspergillus oryzae has a superior capacity to secrete a large amount of proteins into the medium, and is being

industrially used for the production of commercially important proteins. In order to further improve the strain, detailed knowledge about its secretory pathway is crucial. However, current understanding on how elements of A. oryzae’s secretory pathway interact with secreted proteins is still very scarce. Cargo receptor is a class of membrane proteins that are responsible for the efficient recruiting and packaging of cargo proteins into anterograde or retrograde vesicles for trans-organelle transportation. One subclass of cargo receptor, the lectin cargo receptor, specifically interacts with glycoproteins via their lectin domain. In recent years, lines of evidence are implying that lectin cargo receptor not only recruits cargo proteins into transport vesicles but also functions in quality control of secretory proteins. It is then very interesting to investigate the relationship between lectin cargo receptors and heterologous proteins in A. oryzae. The mammalian Vip36 and yeast Emp47p are well-studied lectin cargo receptors, with the former being reported to be involved in post-ER quality control of certain secretory proteins. A BLAST search on the A. oryzae genome database returned two homologs of these two proteins. The two genes ID are AO090026000428 and AO090102000145, which were named Aovip36 and Aoemp47, respectively. Fluorescence microscopy observation revealed that the two lectin cargo receptors mostly colocalized with an early Golgi marker. Deletion of each cargo receptor gene improved the secretion of heterologously expressed bovine chymosin and human lysozyme into the culture supernatant. Moreover, analysis of the ER-enriched fractions revealed that deletion of each gene reduced the degree of heterologous protein accumulation, suggesting that those two cargo receptors may be involved in the retention of heterologous proteins along the secretory pathway. Further studies to investigate the interaction mechanism between those two cargo receptors and heterologous proteins are being conducted. P-20

6 CoA 1 Kenneth Bruno2 1 1 1 1 Scott Baker2 1

1 2

Aspergillus oryzae

CoA CoA

S. cerevisiae A. nidulans

61 1

4

Analysis on the Roles of Six Fatty Acyl-CoA Synthetase Homologs in the Growth of Aspergillus oryzae

Koichi Tamano1, Kenneth Bruno2, Hideaki Koike1, Tomoko Ishii1, Ai Miura1, Myco Umemura1, Scott Baker2,

Masayuki Machida1 (1AIST, 2PNNL)

49 —

P-21 A. oryzae AoRim15

Rim15

Rim15 Aspergillus oryzae Aorim15

Rim15 A. oryzae Aorim15PD

Aorim15 EGFP AoRim15S. cerevisiae A. oryzae

AoRim15 Rim15C Rim15

AoRim15 C

Functional analysis of the stress response regulator protein AoRim15 in Aspergillus oryzae

Hidetoshi NAKAMURA, Takashi KIKUMA, Katsuhiko KITAMOTO

(Dept. of Biotechnol., Univ. of Tokyo) P-22

Aspergillus nidulans

,

188 15P-8

Aspergillus nidulansA. nidulans 12

0.1 3 24 RNA DNA

GO No. 46395 1901575 6631Flavin-containing monooxygenase Cytochrome

P450 AN10582 AN7522

A. nidulans

Analysis of soil humic acid degradation by the fungus Aspergillus nidulans

Nami Nakazawa, Ken-Ichi Oinuma, Naoki Takaya

(Grad. School of Life and Environmental Sciences, Univ. of Tsukuba)

50 —

P-23

Aspergillus oryzaeA. oryzae 50

(Ishitani et al. 1956) A. oryzaeA. oryzae

A. oryzae A. oryzae

A. oryzae (ΔpyrG) (ΔadeB)2

PCR

AoFus3 AoSO

DPY

Investigation of nutrition effects in hyphal fusion efficiency of Aspergillus oryzae

Wakako TSUKASAKI, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO

(Dept. of Biotechnol., The Univ. of Tokyo)

P-24 Aspergillus oryzae 331-25 sense RNA, 331-25 antisense RNA

EST , 331-25 mRNA , 528 EST 43 ,

331-25 sense RNA ( SN RNA) 331-25 antisense RNA ( AS RNA) (1) 331-25 ,

mRNA brlA , PCR

AS RNA , AS RNA , 331-25 331-25 ,

, , 331-25 , , , 331-25

, ( , ) ,, , , 331-25

, 331-25 SN RNA, AS RNA , (1) , 12 p. 46 (2012)

Analysis of 331-25 sense RNA, 331-25 antisense RNA in Aspergillus oryzae

Masaru Tsujii, Hiroto Morita, Hiroshi maeda, Yohei Yamagata, Michio Takeuchi

Dept. of Applied Biological Science, Tokyo Univ. of Agriculture and Technology

51 —

P-25 A. oryzae EcdR

Aspergillus oryzaeMAT1-1 MAT1-2

SclR2

ecdR A. oryzae

A. oryzaeEGFP mDsRed

ecdR 2sclR ecdR

A. oryzae

Promotion effects of deleting a sclerotial formation repressor EcdR for discovery of sexual reproduction in

Aspergillus oryzae

Yuki TANAKA, Feng Jie JIN, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO

(Dept. of Biotechnol., The Univ. of Tokyo) P-26

A. oryzae AoAtg9

/

Atg9 Atg9 Atg

pre-autophagosomal structure(PAS)Aspergillus oryzae

AoAtg9Aoatg9

AoAtg8 EGFPAoatg9

AoAtg9 A. oryzae AoAtg9AoAtg9-EGFP

AoAtg9-EGFP mDsRed-AoAtg8AoAtg9 PAS

Aoatg AoAtg9

Localization and functional analysis of the autophagy-related membrane protein AoAtg9 in Aspergillus

oryzae

Kohei Fujiki, Takashi Kikuma, Katsuhiko Kitamoto

(Dept. of Biotechnol., Univ. of Tokyo)

52 —

P-27

50%

Aspergillus oryzaeAO080569000108

AO080569000108

Congo red Calcofluor whiteGlcNAc EGFP

AO080569000108-EGFPFM4-64 AO080569000108

Functional analysis of functionally-unknown gene related cell wall stress resistance in Aspergillus oryzae.

Nao Tokunaga1, Ayako Senoo3,4, Taiki Futagami2, Kaoru Takegawa2, Kazuhiro Iwashita3,4, Masatoshi Goto2

(1Grad. Sch. Biores. Bioenviron., Kyushu Univ., 2Faculty of Agriculture, Kyushu Univ., 3Grad. Sch. Adv. Sci. Mat., Hiroshima Univ., 4NRIB) P-28

( )

8% (Aspergillus

oryzae)

DNAGeneral amino acid permease (Gap1) (GapA)

1) GapA

PCRGapA

GapA Gap1GapA

GapAGap1

1) 2012

Functional analysis of the Aspergillus oryzae amino acid transporter

Hiromu Hamanaka, Akihiro Kaneko, Harue Kitagawa, Motoaki Sano, Kenji Ozeki, Shinichi Ohashi (KIT)

53 —

P-29 Aspergillus nidulans C sterigmatocystin

Aspergillus nidulans C PKC pkcA

PkcAPkcA cell wall integrity CWI

BckA MkkA MpkA MAPPkcA

sterigmatocystin STaflR mRNA pkcA PkcA

ST STPkcA PkcA ST ST

PkcA STCWI PkcA rlmA

ST MkkA MkkA ST ST PkcA ST

CWI MAP

Functional analysis of Protein kinase C in sterigmatocystin biosynthesis in Aspergillus nidulans

Takuya Katayama and Hiroyuki Horiuchi (Dept. of Biotechnol., Univ. of Tokyo)

P-30 Aspergillus nidulans CON

a b Özlem Sarikaya Bayrama Özgür Bayrama Gerhard H. Brausa

a b 1980

con con-6 con-10CON-6 CON-10

96 86CON-6 CON-10

conF conJ GFPFM4-64

Role of CON-6 and CON-10 homologue in Aspergillus nidulans

Satoshi Suzukia b Özlem Sarikaya Bayrama Özgür Bayrama Gerhard H. Brausa

(a Georg-August-Universität Göttingen b NFRI)

54 —

P-31 Aspergillus nidulans

,

Aspergillus nidulans AmyR

AmyR STAmyR ΔamyR

AmyR A. nidulans

ΔamyRDNA ΔamyR ST PKS

NRPSAmyR AmyB ΔamyB

ΔamyRgphA ΔgphA ΔamyB

AmyB GphAA. nidulans GphA AmyB

ΔamyR STST

Analysis for glycogen and sterigamtocystin metabolisms by Aspergillus nidulans

Airi Komatsuzaki, Yosuke Kamimura, Naoki Takaya

(Grad Sch Life Environment Sci, Univ. of Tsukuba) P-32

HECT HulA

MalP

Rsp5CreD

MalP 2012Rsp5 HulA MalP

HulA 3 WW CreD-3FLAG GST-HulA-WW domainCreD HulA WW

HulAsGFP-MalP HulAsGFP-MalP HulA MalP

HulA WW CreDPPLY 3 PXY CreD-HulA

Involvement of HECT ubiquitin ligase, HulA, in degradation of maltose permease in Aspergillus oryzae

Yuka Matsuura, Mizuki Tanaka, Tetsuya Hiramoto, Takahiro Shintani, Katsuya Gomi

(Div.Biosci.Biotechnol.Future Bioind.,Grad.Sch.Agric.Sci.,Tohoku Univ.)

55 —

P-33 (O-1)

1 1 1 1 2 1 1 2

Virocontrol

Propidium iodideFUN-1

RNAseqresponse to stimulus, transport

Cytological and transcriptional analyses of heterogenic incompatibility in Rosellinia nacatrix. 1Takahiro Uwamori, 1Kanako Ikeda, 1Chiaki Kida, 1Hitoshi Nakayasiki, 2Satoko Kanematsu, 1Kenichi Ikeda

(1Grad. Sch. Agric. Sci, Kobe Univ. 2Nat. Inst. Fruit Tree Sci. NARO)

P-34

A. oryzae 2 acyl-CoA binding protein (AoAcb1 AoAcb2)

acyl-CoA binding protein (Acb1) Acb1 Acb1

ACBP (Acyl-CoA Binding Protein) Acb1 ACBP

2 Aspergillus oryzae 2 acyl-CoA binding protein (AoAcb1 AoAcb2)

AoAcb1-EGFPAoAcb2-EGFP Aoacb2 thiA

Aoacb2Aoatg1 Aoatg4 Aoatg8

AoAcb1Aoatg15 AoAcb1

AoAcb1Aoacb2 AoAcb1 AoAcb2

Analysis of two acyl-CoA binding proteins (AoAcb1, AoAcb2) in Aspergillus oryzae

Kouhei KAWAGUCHI, Takashi KIKUMA, Katsuhiko KITAMOTO

(Dept. of Biotechnol., Univ. of Tokyo)

56 —

P-35 (O-2) A. oryzae AoSO

Aspergillus oryzae

A. oryzaeAoSOAoSO

AoSO 3 N 1-555C 1147-1195

AoSO C N

AoSOAoSO 2

AO090005001564 AosoAO090005001564 AoBni1

AoSO AO090005001564

Investigation of AoSO function in cell repair after hyphal wounding in Aspergillus oryzae

Junpei KAWABATA, Kei SAEKI, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO

(Dept. of Biotechnol., The Univ. of Tokyo)

P-36 Aspergillus oryzae

hydrophobin70~150a.a.

Aspergillus oryzae hypA,B,C

hypA,B,C , HypA HypB HypChypA hypB

HypA HypBhypB hypA hypA hypB

eGFP, DsRed hypA, hypBHypA, HypB

HypA hypAhypA ,hypA -400~-200bp

Characterization and localization of hydrophobins in Aspergillus oryzae

Yui Yamakawa, Chie Ishida, Fuyuka Hayakawa, Yuka Mizuno, Harushi Nakajima

(Dept. of Agricultural Chemistry, Univ. of Meiji)

57 —

P-37 Aspergillus oryzae

, ,

Aspergillus oryzae

Polyphosphate metabolism and stress response in Aspergillus oryzae

Sawaki Tada, Hikaru Ohkuchi, Ken-Ichi Kusumoto (NFRI, NARO) P-38 Roles of AoLAH in regulating Woronin body position and function in Aspergillus oryzae Pei HAN, Feng Jie JIN, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO (Dept. of Biotechnol., The Univ. of

Tokyo) Woronin body is a Pezizomycotina-specific organelle observed in the vicinity of septum, and plugs the septal

pore in response to cellular wounding. Previously, we characterized AoHex1 as the major protein of Woronin body in Aspergillus oryzae1), and investigated the Woronin body differentiation from the peroxisome2). Recently, the Leashin (LAH) protein was identified to tether Woronin bodies to the cell cortex in Neurospora crassa3). In this study we attempted to investigate the roles of Leashin protein in regulating Woronin body position and function in A. oryzae.

We predicted Aolah gene encoding a single polypeptide of 5,727 amino acids, which is homologous to the N. crassa lah gene expressed into two distinct proteins3). AoLAH-EGFP expressed from the endogenous locus was detected as Woronin body-like dots along the hyphae and closely associated with the septum. Transmission electron microscopic analysis demonstrated that the Aolah gene disruption led to no tethering of Woronin bodies in the vicinity of septum. We also observed excessive loss of the cytoplasm upon hyphal injury in Aolah disruptant, suggesting the functional involvement of AoLAH in the septal plugging ability of Woronin body.

N-terminal conserved region of AoLAH (AoLAH[1-2039]) was expressed as EGFP fusion protein, and it was detected at both sides of the septal pore, which is typical for Woronin body localization, suggesting a role of AoLAH N-terminal region in association with Woronin body. C-terminal conserved region of AoLAH (AoLAH[4710-5727]) expressed as EGFP fusion protein was detected closely to the septal pore both in the wild-type strain and Aolah disruptant, indicating that AoLAH[4710-5727] itself functions for associating to the septum. When AoLAH N- and C-terminal fusion fragment lacking the unconserved middle region was expressed in Aolah disruptant, Woronin bodies were re-discovered in the vicinity of septum, however, the cytoplasm was still excessively lost upon hyphal injury. This suggests that the unconserved middle region of AoLAH has a role in the septal plugging ability of Woronin body, which is being further evaluated. 1) Maruyama et al. (2005) Biochem. Biophys. Res. Commun. Vol. 331, 1081-1088. 2) Escaño et al. (2009) Eukaryot. Cell Vol. 8, 296-305. 3) Ng et al. (2009) PLoS Genet. Vol. 5, e1000521.

58 —

P-39 DNA

DNA DNADNA NHEJ

DNANHEJ

SSA MMEJNHEJ SSA MMEJ

TAIL-PCRDNA

The mode of random integration of exogenous DNA in Neurospora crassa

Ryuhei Kamada, Shuuitsu Tanaka, Shin Hatakeyama

(Lab. Genet., Dept. Regulatory Biol., Saitama Univ.) (1Division Appl. Biol., Fac. Tex. Sci. Tech., Shinshu Univ., 2Mushroom Lab. Hokuto Co.)

P-40

A. oryzaeAoAtg26

Atg26Pichia pastoris PpAtg26

Sccharomyces cerevisiae atg26A. oryzae AoAtg26

A. oryzae Aoatg26

A. oryzaeAoAtg26

EGFP-AoAtg8 Aoatg26Cvt AoApe1-EGFP

Aoatg26 CvtAoAtg26

Aoatg26

Analysis of the autophagy-related sterol glucosyltransferase AoAtg26 in Aspergillus oryzae Takashi Kikuma, Takayuki Tadokoro, Katsuhiko Kitamoto

(Dept. of Biotechnol., Univ. of Tokyo)

59 —

P-41

Effects on growth and repression of A.oryzae GapA gene disruption.

Akihiro Kaneko, Hiromu Hamanaka, Motoaki Sano, Kenji Ozeki, Shinichi Ohashi

(Kanazawa Institute of Technology)

P-42 MOA

Aspergillus oryzae 1)

4-methyl-2-oxopentanoic acid (MOA)

MOA 2-

5MOA NAD(P)H MOA

5 1 NAD(P)H MOA TEF1

MOA 1) Suzuki, M. Yoneyama, H. Koizumi, T. (1982) The mechanism of formation of ethyl leucinate, an important aroma component in sake. Hakkokogaku, 60, 19-25.

Overexpression and characterization of a novel 4-methyl-2-oxopentanoic acid (MOA) reductase in Aspergillus

oryzae.

Tatsuya Yamamoto, Emiri Koide, Tiaki Mori, Takeura Kengo, Miki Daikoku Motoyuki, Shimizu, Masashi Kato

(Fac. of Agri., Meijo Univ.)

60 —

P-43 (O-9) Trichoderma reesei 1 2 1 3 3 4 1

1 2 3 4

Trichoderma reesei

FT-IR

T. reesei6

GPIT. reesei GPI

Exploration of protein related to cell surface fibrous material synthesis of Trichoderma reesei

Shingo Tahara1, Mikiko Nitta2, Yosuke Shida1, Yoshiki Horikawa3, Junji Sugiyama3, Masako Osumi4, Wataru

Ogasawara1

(1Nagaoka Univ. of Technol., 2JST, 3RISH, Kyoto Univ., 4IIRS)

P-44

Poly(ADP-ribose) glycohydrolase 1 1 2 2 1 1 1 2

(ADP- ) (ADP-

) poly(ADP-ribose) polymerase (PARP) poly(ADP-ribose) glycohydrolase (PARG) 2 PARPDNA Aspergillus nidulans

parp ortholog 1 A. nidulans parg ortholog

A. nidulans 7 parg 1 Mg2+

(ADP- ) fungal PARG (fPARG) A. nidulans DNA (MMS) parp fparg

fPARG DNA fparg

Identification and Characterization of the Novel Poly(ADP-ribose) Glycohydrolase of Filamentous Fungus

Aspergillus nidulans.

Mio Hirano1, Miho Sudo1, Shunsuke Masuo2, Naoki Takaya2, Motoyuki Shimizu1, Masashi Kato1 (1Fac. Of Agri.,

Meijo Univ., 2Grad. Sch. of Life & Env. Of Tsukuba)

61 —

P-45 Aspergillus oryzae ADAM

, ,

Aspergillus oryzae , ADAM ( A Disintegrin And Metalloproteinase ) 2 , 2

admA, admB ADAM , , ADAM 30

ADAM , , , ADAM , A. fumigatus ADM-B

sheddase Schizosaccharomyces pombe Mde10 ,

, A. oryzae ADAM admA, admB ∆admA ∆admB , ∆admA∆admB

, , calcofluor white congo red ,

37 , ,

, A. oryzae ADAM

Functional analysis of intracellular metalloprotease ADAM in Aspergillus oryzae

Takuji KOBAYASHI, Hiroshi MAEDA, Michio TAKEUCHI, Youhei YAMAGATA

(Dept. of Applied Biological Chemistry, Tokyo Univ. of Agriculture and Technology) P-46

hydrophobin RolA 1 1 2 3 4 1,2 1 2

3 4

hydrophobin RolA PBSA PBSACutL1 PBSA hydrophobin kinetics

RolA Leu137, Leu142 PBSA pH4-10 PBSARolA pH4 PBSA pH4-10 0

RolA pI=4.6-4.8 PBSA RolA

RolA RolA QCMpH4, 7, 10

RolApH4, 7 pH10 RolA

RolARolA pH4

RolA RolA

RolA kinetics

Analysis of the interaction between Aspergillus oryzae hydrophobin RolA and solid surfaces

Takumi Tanaka1, Hiroki Tanabe1, Toru Takahashi2, Takanari Togashi3, Toshihiko Arita4, Keietsu Abe1,2 (1 Grad. Sch.

Agric. Sci., Tohoku Univ., 2 NICHe., Tohoku Univ., 3 Dept. Sci., Yamagata Univ., 4 IMRAM., Tohoku Univ.)

62 —

P-47

Aspergillus oryzae 3 pepstatin insensitive protease (PIP) 317-062315-005 306-018 PIP

DGDT W(F/Y)EW(F/Y/L)P ED(F/Y) PIP aspartic protease

glutamic protease A. oryzae PIP

PIP 3 mRNA317-062 315-005 Pichia pastoris 306-018

Aspergillus nidulans 317-062 306-018315-005 8 M urea

3 PIP pH317-062 306-018 pH pH

315-005

Enzymatic Properties of Pepstatin Insensitive Protease in Aspergillus oryzae

Marii Takeuchi, Ayako Okamoto, Haruka Abo, Hiroshi Maeda, Youhei Yamagata, Michio Takeuchi

(Division of Applied Biological Chemistry, Tokyo Univ. of Agriculture and Technology)

P-48 (O-10) 3D

Coleophoma empetri F-11899FR901379 A AAC

Micafungin AAC

AAC

AAAC A

CAPCR Pseudomonas sp. SY-77 CA

AAAC

Substrate switching of antibiotics acylase by 3D modeling Kazuki Nakayama, Masato Yamada, Hiromi Nishida, Yasuhiro Isogai (Toyama Pref. Univ., Dept. Biotech.)

63 —

P-49

4

chymosin chymosin - casein Phe105 – Met106Aspergillus oryzae

A. oryzae RIB40 11

1

AOchymosin 1 A. nidulans A89AOchymosin 1

pH 30 pH 5.5 A. luchuensisALchymosin 1

Characterization of milk clotting enzymes in Aspergilli

Yoko Takyu, Ayako Okamoto, Hiroshi Maeda, Yohei Yamadata, Michio Takeuchi

( Division of Applied Biological Chemistry, Tokyo Univ. of Agriculture and Technology ) P-50 Aureobasidium pullulans -4-

Aureobasidium pullulans ATCC 20524-L- abfB DNA 4,996 bp

DNA abfB -4-(bphA) A. pullulans ATCC 20524 cDNA

RACE (Rapid Amplification of cDNA Ends) bphA

A. pullulans ATCC 20524 30 3mRNA cDNA cDNA RACE

5� RACE 3� RACE bphA PCRabfB 555 bp bphA ORF 1,512 bp

54 bp 1 bphA 96 bp TATA63 bp 114 bp A bphA

bphA cDNA ORF EcoRI NotIpET-26b

pBPH103 E. coli BL21 (DE3) bphA cDNA BphA

Characterization of benzoate-4-hydroxylase gene from Aureobasidium pullulans.

Chihiro Higashida, Kazuyoshi Ohta

(Dept. Biochem. Appl. Biosci., Univ. of Miyazaki)

64 —

P-51 Aspergillus nidulans GfsA O- Galƒ

1 2 1 2 2 1 1 1 2

, Aspergillus nidulans GfsA (Galƒ)

, GfsA O- Galƒ∆gfsA (GMP) ,

GfsA-3xFLAG UDP-Galƒ , EB-A240-200 kDa GMP PNGase F

-elimination , PNGase F-elimination , O- pmtA pmtC

GMP wt , O-WscA-HA wt, ∆gfsA , WscA-HA

HA EB-A2 , ∆gfsA WscA-HA EB-A2, ∆gfsA WscA-HA GfsA-3xFLAG

in vitro Galƒ , WscA-HA Galƒ, GfsA O- Galƒ

GfsA is a novel glycosyltransferase involved in biosynyhesis of galactofuranose antigen of O-glycan in

Aspergillus nidulans.

Haruka Motomatsu1, Taiki Futagami2,Keisuke Ekino1, Kaoru Takegawa2,Masatoshi Goto2,Yoshiyuki

Nomura1,Takuji Oka1(1 Univ. of Sojo,2Univ. of Kyusyu)

P-52

1- -3-

LOXN001 2 LOX

PoLOX1 PoLOX2 LOXPoLOX1 PoLOX2 cDNA PoLOX1 PoLOX2

PCR PoLOX1 PoLOX2 cDNA

PoLOX1 PoLOX2 640 72 kDa 661 74 kDa1 PoLOX1 PoLOX2 cDNA pET16b

BL21(DE3) SDS-PAGE72 kDa 74 kDa pH PoLOX1

pH 11.5 PoLOX2 pH 5.5 7.0 PoLOX1 PoLOX2 Km Vmax0.05 mM 0.22 µmol/min 0.18 mM 0.90 µmol/min

Functional analysis of two lipoxygenase genes of Pleurotus ostreatus strain N001

Daiki Kobayashi, Kenta Oguro, Yuji Tasaki

(Dept. of Mater. Eng., Nagaoka Col. of Technol.)

65 —

P-53 Aspergillus oryzae D-

(DamA) 1 1 1 1, 5 1 1 2

3 4 4 1 1 2 3 4

5JIRCAS

β- (BapA) N β- (β-Ala )(Heck, T et, al. 2007, 2012) BapA

(AO090138000075) Aspergillus oryzaeA. oryzae N His-Tag

17 (L 13 , D 3 , β 1) aminoacyl-p-nitroanilide (pNA) β-Ala-pNA BapA D-Leu-pNA

D-Phe-pNA D-stereoselective aminopeptidase (DamA)D-Leu-NH2 LC/MS/MS D-Leu-OH

D-Leu-D-Leu-NH2 DamA BapAD-Leu-pNA 3.0 M NaCl DamA pNA 3.1

DamAM. Matsushita-Morita et al.

Appl. Biochem. Biotechnol. (in press) Characterization of a D-stereoselective aminopeptidase (DamA) from Aspergillus oryzae Mayumi Matsushita-Morita1, Hiroyuki Nakagawa1, Sawaki Tada1, Junichiro Marui1,5, Satoshi Suzuki1, Ryota Hattori1, Hitoshi Amano 2, Hiroki Ishida3, Youhei Yamagata4, Michio Takeuchi4, Ken-Ichi Kusumoto1

(1 Natl. Food Res. Inst., NARO, 2Amano Enzyme, 3Gekkeikan, 4Tokyo Univ. of Agric. Tech., 5 JIRCAS.)

P-54 Aspergillus oryzae pepO

Aspergillus oryzae RIB 40

126 RIB 40 APase PepO

pepO pyrG pepO

pepO pepO pepORIB 40 5

APasepepO antipain

antipain AorsinAZ-Arg-Arg-MCA RIB 40 pepO aorsinA

RIB 40PepO AorsinA

Extracellular endoproteases of Aspergillus oryzae pepO gene deletion strain

Ayako UENO , Hiroshi MAEDA , Youhei YAMAGATA , Michio TAKEUCHI (Division of Applied Biological Chemistry,Tokyo Univ. of Agriculture and Technology)

66 —

P-55 Aspergillus oryzae saccharolysin homolog

( ) [ ] A. oryzae S. cerevisiae 1 saccharolysin homolog oryzalysin ( Ols ) A , B , C Saccharolysin

A. oryzae ols A , ols B , ols C 3 A. oryzae Ols A. oryzae

oryzalysin [ ] ols A , ols B , ols C

ols A , ols B , ols C 2 , 4 – dinitrophenol olsA

olsA ols A , ols B , ols C olsC

olsA olsC olsA olsA olsC

Functional analysis of intracellular metalloproteases saccharolysin homolog in Aspergillus oryzae

Yuichiro ISHIKAWA,Hiroshi MAEDA,Michio TAKEUCHI,Youhei YAMAGATA (Division of Applied Biological Chemistry, Tokyo Univ. of Agriculture and Technology)

P-56 (O-7) Trichoderma reesei 10 β-glucosidase isozyme

Trichoderma reesei β-glucosidase (BGL) BGL

10 BGL (Cel1A Cel1B Cel3A Cel3B Cel3C Cel3D Cel3E Cel3F Cel3G Cel3H)

GH1 Cel1A Cel1B GH3 BGL

BGL p-Nitrophenyl-β-D-glucoside (pNPG1) (G2) Cel3H BGL BGL

pNPG1 G2 Cel3A Cel3BCel3E Cel3C Cel3D Cel3F Cel3G

Cel1B pNPG1 G2 Cel1A G2 BGL 10% G2 pNPG1

BGL G2 BGL (Cel1 Cel3ACel3B Cel3E)

Cel1A Cel3A5.4 mg/ml 2.8 mg/ml Cel3A

Cel1A Cel1A

Enzymatic properties of β-glucosidase isozymes from Trichoderma reesei

Boyang Guo, Kouichi Nozaki, Masahiro Mizuno, Yoshihiko Amano

(Dept. of Biosci. & Textile Technol., Interdisciplinary Graduate School of Sci. and Technol., Shinshu Univ.)

67 —

P-57

Tricholoma matsutakeL- Phe

PhePhe PAL PAL

TmPAL1 cDNA DNA PALTmPAL1

PAL NBRC30605 RNA RT-PCR 3 5 RACE TmPAL1 cDNA

TmPAL1 PAL 60%TmPAL1 PCR 3 5 PCR

cDNA 74 DNA TmPAL1

pET-28 BL21(DE3)TmPAL1

Structure and functional characterization of a phenylalanine ammonia-lyase gene, TmPAL1, in Tricholoma

matsutake.

Hayato Miyakawa, Naoki Katsuda, Yuji Tasaki

(Dept. of Mater. Eng., Nagaoka Col. of Technol.)

P-58

hydrophobin RolA cutinase CutL1 RolA

1, 1, 1, 1, 1, 2, 3, 1

1 2 3

hydrophobin , Aspergillus oryzae, polybutylene succinate-co-adipate(PBSA)

hydrophobin RolA PBSA cutinase CutL1 PBSA RolACutL1 , PBSA CutL1 PBSA

, RolA CutL1 , RolA His32, Lys34 CutL1 Glu31, Asp142, Asp171 , RolA , H32S/K34SCutL1 , E31S/D142S/D171S , NaCl

, RolA CutL1 NaCl

, RolA CutL1RolA

RolA , QCM RolA-CutL1 , RolA His32, Lys34 Lys41, Lys46, Lys51 , His32, Lys34

CutL1 , Kd

Aspergillus oryzae hydrophobin RolA possesses positive residues involving in RolA-cutinase Cutl1 interaction

Yusei Tsushima1, Daiki Sato1, Yoonkyung Kim1, Kimihide Muragaki1, Kenji Uehara1, Toru Takahashi2, Youhei

Yamagata3, Keietsu Abe1 (1 Grad. Sch. Agric. Sci., Tohoku Univ., 2 NRIB., 3 Tokyo univ. of Agric. And Technol)

68 —

P-59

1 1, 2, 2, 2, 2, , 1 1, 1 1 , 2

Aspergillus kawachii

25 40 3040 RNA DNA Agilent

Technologies 2296 250 Gene Ontology

40 30

Transcriptome analysis of white koji mold in a barley koji-making process

Taiki Futagami1, Kazuki Mori1, Shotaro Wada2, Yasuhiro Kajiwara 2, Hideharu Takashita2, Toshiro Omori2,

Kosuke Tashiro1, Satoru Kuhara1, Masatoshi Goto1

(1Kyushu Univ., 2Sanwa Shurui Co., ltd.) P-60

Phanerochaete chrysosporium

Phanerochaete chrysosporium

2% w/v 4% w/v2% w/v Cel7

4% w/v

9 Cel7 14

Static cultivation of Phanerochaete chrysosporium to unveil responses to xylan components

Yuki Nakamura, Chiaki Hori, Kiyohiko Igarashi, Masahiro Samejima

(Dept. Biomat. Sci., Univ. of Tokyo)

69 —

P-61 CreA CreB

CreA Aspergillus nidulans CreA

CreB CreA creA, creB

creA/creB creA creA/creB creB

-

creA creA/creB - creA/creB

creA -

Hyperproduction of amylases by double deletion of CreA and CreB involved in carbon catabolite repression

in Aspergullus oryzae

Sakurako Ichinose, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi

(Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ.) P-62

AmyR::XlnR

XlnR

AmyR N

FLAG-His-tag AmyR DNA N 68aa XlnR DNA

C 812aa AmyR::XlnR amyR

FLAG AmyR::XlnR

AmyR::XlnR CMC

A model chimeric transcription factor: construction and characterization of an AmyR::XlnR hybrid

transcription factor.

Yuhei KANEKO1, Hisaki TANAKA2, Jun-ichiro MARUI , Motoyuki SHIMIZU1, Tetsuo KOBAYASHI2, Masashi

KATO1. 1Meijo Univ., 2 Nagoya Univ.

70 —

P-63 (O-6) Trichoderma reesei

Trichoderma reesei

PC-3-7 MFSTRP3405 PC-3-7

TRP3405PC 3405 PC-3-7

PC 3405PC 3405 PC-3-7

Influence of gene destruction of cellulose specific transporter in Trichoderma reesei

Hiroki Taniguchi, Hideyuki Kusaka, Tkanori Furukawa, Yosuke Shida, Wataru Ogasawara

(Nagaoka Univ. of Tech)

P-64 Aspergillus nidulans ManS

A. oryzae ManRA. nidulans manR manS manR manS

manS , manS konjac glucomannan (KG) 24 ,

RNA RNA manC, manE, manF ManS ManR manB manC

manC ManR ManS ManRManS manC eglA eglB cbhA cbhD

faeC ManR ManSCMC manS EglA EglB ManS

ManRKG manSNorthern

Physiological function of ManS, a fungal-specific transcription factor in Aspergillus nidulans

Ayako Watanabe, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi

(Graduate Sch. of Bioagric. Sci., Nagoya Univ. )

71 —

P-65

1 1 1 2 1 1 1

(1 2 )

Ca2+ CaM

Phanerochaete chrysosporium

Pleurotus ostreatus Lac

MnP PC9 CaM

shRNA Lac MnP

CaM W-7 Lac MnP

CaM

Lac MnP

Role of calmodulin in regulation of ligninolytic enzymes expression in Pleurotus ostreatus.

Takashi Suetomi1, Takaiku Sakamoto1, Yoshitaka Tokunaga1, Yoichi Honda2, Kousuke Izumitsu1, Kazumi Suzuki1

and Toshikazu Irie1

(1Unvi. of Shiga Pref., 2Kyoto Univ.)

P-66 Aspergillus aculeatus

Aspergillus aculeatus

cellobiohydrolase I (cbhI) orotidine 5’-phosphate

decarboxylase T-DNA 5-fluoroorotic acid (5-FOA) 5-FOA

cbhI cbhI (B7 ) inverse PCR B7 T-DNA

pPTRII B7 (gene1) cbhI gene1

Identification of regulator(s) controlling the expression of cellulase genes in Aspergillus aculeatus

Syota Yuki, Emi Kunitake, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi

(Grad. Sch. Life & Env. Sci., Osaka Pref. Univ.)

72 —

P-67 Expression cluster analysis of the transcriptomes at sequential growth stages in the white-rot fungus, Phanerochaete carnosa, grown on aspen and spruce woods. Hitoshi Suzuki (RIKEN, CSRS), Philip Wong, Chi-Yip Ho, Yunchen Gong, Kin Chan, Elisabeth Tillier and Emma Master (Univ. of Toronto)

A white-rot fungus, Phanerochaete carnosa, is a natural decomposer of coniferous wood and has an ability to degrade various types of softwood and hardwood tissues. While proteins produced by P. carnosa are expected to comprise promising enzymes for bioprocessing lignocellulose including fibre from coniferous wood, genome sequencing revealed that a large contingent of likely relevant proteins remain uncharacterized. Here, we performed gene expression cluster analysis using multiple P. carnosa transcriptomes obtained at different stages of fungal growth on trembling aspen and white spruce. The results revealed co-expression pairs and clusters of known lignocellulolytic genes associated with genes of unknown functions (UNKs), suggesting participation of those unknowns in the lignocellulolytic system of this fungus.

P-68

AmyR MalR

AmyR

MalR 2

AmyR MalR 1AmyR MalR

AmyR MalR thiA AmyR

MalR

AmyR MalRMalR AmyR

AmyR phosphatasePhos-tag SDS-PAGE

1 12 p.57

Post-translational modification and stability of transcription factors, AmyR and MalR, in Aspergillus oryzae.

Kuta Suzuki, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi (Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ)

73 —

P-69 pH PacC

1 2 1 1 1 1 1 2

Aspergillus nidulans pH PalC

PalC C2H2 PacCPalC PacC

pacC pH (pH 4.2) (pH 8.2)

qRT-PCRΔpacC

eglAPacC pH PacC eglA

qRT-PCRPacC

RNA-seq ManRΔpacC PacC

TLC ΔpacCPacC ManR

pH

The pH-signaling transcription factor PacC regulates expression of cellulase genes in Aspergillus nidulans.

Emi Kunitake1, Daisuke Hagiwara2, Kentaro Miyamoto1, Kyoko Kanamaru1, Makoto Kimura1, Tetsuo Kobayashi1

(1Graduate Sch. of Bioagric. Sci., Nagoya Univ., 2MMRC, Chiba Univ.)

P-70

xyrA larA XlnR AraR

XlnR AraR Aspergillus Zn(II)2Cys6

xyrA larA XlnR AraR

XlnR AraR DNA xyrA larADNA EMSA xyrA XlnR 2

5’-GGCTAA-3’ AraR 5’-CGGGTAAA-3’60 bp XlnR AraR 11 bp larA

AraR 3 XlnR 5’-GGATAA-3’1 AraR 40bp XlnR

AraR XlnR AraRXlnR AraR

xyrA larA

Co-regulation of A. oryzae xyrA and larA expression by XlnR and AraR

Kana Ishikawa, Tomomi Kawase, Yuji Noguchi, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi

(Graduate Sch. of Bioagric. Sci, Nagoya Univ)

74 —

P-71 Aspergillus aculeatus ClbR ClbR2

Aspergillus aculeatus cellobiose

response regulator (ClbR) XlnR ClbR

ClbR XynIa Yeast Two Hybrid (Y2H)

ClbR ClbR 42% ClbR2 C (ClbR21-295) clbR2 clbR

ClbR2 ClbR ManR ClbR FIa-xylanase (xynIa), FIb-xylanase (xynIb)

ClbR in vitro DNA ClbR (MalE-ClbRwhole)

xynIa Electrophoresis mobility shift assayY2H ClbR ClbR2 C

(MalE-ClbR21-295) ClbR ClbR2 xynIa manR

Functional analysis of cellobiose response regulator (ClbR) and its paralog (ClbR2) in Aspergillus aculeatus.

Ayano Kawamura, Emi Kunitake, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi

(Grad. Sch. Life & Env. Sci., Osaka Pref. Univ.) P-72 Molecular basis that divides ManR-dependent and ManR/McmA-dependent genes in Aspergillus nidulans Nuo Li, Miki Aoyama, Kyoko Kanamaru, Makoto Kimura, Tetsuo Kobayashi

(Grad. Sch. Bioagric. Sci., Nagoya Univ.) Fungal hydrolytic gene expression is tightly controlled at the transcription level. Besides the traditional XlnR-dependent mechanism, a novel McmA-ManR mechanism involved in regulation of cellulolytic and hemicellulolytic genes has been identified recently. Both transcription factors are essential to inductive expression of eglA, encoding endoglucanase A, because ManR requires McmA for binding to CeRE (Cellulose Responsive Element) on the promoter. Based on RNA sequencing analysis, highly expressed cellulolytic genes are under control by both McmA and ManR (ManR/McmA dependent). But at the same time a number of genes are ManR-dependent but not McmA-dependent (ManR-dependent). The ManR-dependent genes include some mannanolytic and xylanolytic genes. In this report, we confirmed the requirement of McmA for ManR-binding using the promoter of eglB, another endoglucanse gene, and also examined whether ManR without McmA could bind the promoter of the ManR-dependent gene mndB encoding β-mannosidase. Binding of His-tagged McmA and Flag-tagged ManR DNA binding domain to various probes were examined by EMSA. When binding to the eglB was examined results similar to eglA were obtained. ManR did not bind to the promoter in the absence of McmA, while it bound to CeRE containing DNA fragments in its presence. McmA alone could bind to various locations on the promoter, but CeRE was required for recruitment of ManR. This implies ManR not only binds to McmA but also to DNA. In contrast, ManR could bind to the mndB promoter in the absence of McmA. Since no CeRE-like sequence exists on the promoter, it appeared that ManR recognized a sequence different from CeRE. Further study will focus on the identification of the binding site for ManR on the mndB promoter. This work was supported by the Programme for Promotion of Basic and Applied Research for Innovation in Bio-oriented Industry.

75 —

P-73 Aspergillus niger PKS-NRPS

Aspergillus niger polyketide synthase – non-ribosomal peptide synthase (PKS-NRPS)

PKS-NRPS An11g00250 (pynA) (pyn ) PynpynR PynR GAL4

A. niger ATCC 1015 PEG- pynRgpdA promoter pynR

pyn RT-PCRpynR pynA

pynaga pynR

NMR MS pyranonigrin E

Expression of the dormant PKS-NRPS gene cluster in Aspergillus niger

Takayoshi Awakawa, Xiao-Long Yang, Toshiyuki Wakimoto, Ikuro Abe

(Grad. School of Pharm. Sci., Univ. of Tokyo)

P-74

Tri6

Fusarium graminearum

Tri Tri6Tri10 Tri5

Tri4 Tri6 Tri TRI6Tri6

Tri6 Tri6_nsm Tri6_nsm Tri6Tri6_nsm Tri6_nsm

Tri6_nsm TRI6Tri6::GFP Tri6_nsm

Tri6 TRI10 TRI6 zinc finger GFPTRI10 TRI6 TRI10

Tri Tri6Tri TRI6

Functional analysis of Tri6 that encodes a transcriptional factor for trichothecene biosynthesis. Yuichi Nakajima, Yoshiyuki Kito, Kazuyuki Maeda, Hinayo Ichikawa, Tetsuo Kobayashi, Makoto Kimura (Graduate Sch. of Bioagric. Sci., Nagoya Univ.)

76 —

P-75

ACATE Eg5

E

Chaunopycnis alba RK99-F33

C6

LaeA p38 MAPKLaeA Lae1

LaeA

Production control mechanism of terpendole mycotoxins

Takayuki Motoyama, Hiroyuki Osada

(Antibiotics Lab., RIKEN) P-76

1 DMSO

p38 MAP OSM1LaeA

LaeA LAE1 DMSODMSO p38 MAP

OSM1 LAE1DMSO p38 MAP LAE1

Bioactivity of tenuazonic acid and its production control mechanism in Magnaporthe oryzae.

Choong-Soo Yun, Takayuki Motoyama, Yusi Hutamura, Hiroyuki Osada

(Antibiotics Lab., RIKEN)

77 —

P-77 (O-3) Aospt3

1,2 1,2 1,2 1 2

Aospt3secondary metabolites SMs

SMs Aospt3 SMs SMs ΔAospt3

(kojA kojR kojT) 100Aospt3

SMs laeAAospt3 laeA ΔAospt3 laeA

ΔAospt3ΔlaeAΔlaeA

Aospt3 laeA ΔlaeAspt3 ΔAospt3 laeA

Aospt3 laeA

Functional analysis of Aospt3 secondary metabolic regulator

Masato Hirose , Moriyuki Kawauchi, Kazuhiro Iwashita

(Hiroshima Univ, NRIB)

P-78 Emericella variecolor

Emericella variecolor II

variecolin ophiobolin astellatol variculanolE. variecolor

E. variecolor Aspergillus

clavatus ophiobolin F synthase (AcOS) 3 AcOS

Phomopsis amygdali fusicoccadiene synthase

Aspergillus oryzae

The search for sesterterpene synthases from Emericella variecolor Takaaki Mitsuhashi, Yudai Matsuda, Takayoshi Awakawa, Ikuro Abe

(Graduate School of Pharmaceutical Sciences, Univ. of Tokyo)

78 —

P-79 anditomin

Aspergillus variecoloranditomin [2,2,2]Diels-Alder

anditomin anditominDiels-Alder Diels-Alderase

anditomin 13

25

5 Aspergillus oryzae

7 5Diels-Alderase

Biosynthetic studies on a fungal meroterpenoid anditomin

Yudai Matsuda, Takayoshi Awakawa, Ikuro Abe

(Graduate School of Pharmaceutical Sciences, Univ. of Tokyo) P-80 Aspergillus fumigatus pseurotin A

Pseurotin A Aspergillus fumigatus immunoglobulin

E

2007Turner A. fumigatus polyketide synthase - non-ribosomal peptide synthetase (Afu8g00540) pseurotin A

Aspergillus oryzaepTAex3 pUSA pAdeA pPTRI

AmyB Afu8g00540 hydrolase (Afu8g00530 Afu8g00570) P450(Afu8g00560) methyl transferase (Afu8g00550) A. oryzae NSAR1

Functional analysis of pseurotin A biosynthetic gene cluster in Aspergillus fumigatus

Koichiro Shiomura, Yudai Matsuda, Takayoshi Awakawa, Ikuro Abe

(Grad. School of Pharm. Sci., Univ. of Tokyo)

79 —

P-81 Fusarium sp. FN080326 A

1 Jun-Pil JANG2 Jae-Hyuk JANG2 1 Jong Seog AHN2 1

1 CSRS 2KRIBB

A Fusarium sp. FN080326Fusarium

PKS-NRPS

FN080326

PKS-NRPS 1eqxS Kakule TB, et al. 2013 ACS Chem Biol

8:1549-1557 eqxfsa

fsa P450 fsa9

Identification of fusarisetin A biosynthetic gene cluster of Fusarium sp. FN080326 Naoki Kato1, Jun-Pil Jang2, Jae-Hyuk Jang2, Shunji Takahashi1, Jong Seog Ahn2, Hiroyuki Osada 1 (1RIKEN CSRS, 2KRIBB)

P-82 Fusarium sp. RK97-94

luc1 luc4 1,2, 1, 1, 2, 2 1 2

(1) Fusarium sp. RK97-94

p53 1NG-391(2) 1 2 PKS-NRPS luc5

8 1luc1 luc4

luc4 2

1 Luc4 1

1 2 Luc4luc1 1

2 1 (3) Luc12

1

Functional analysis of the methyltransferase gene luc1 and the transporter gene luc4 in the lucilactaene

biosynthesis gene cluster of the producing fungus Fusarium sp. RK97-94

Sho Kato1,2, Takayuki Motoyama1, Hiroshi Hirota1, Takashi Kamakura2, Hiroyuki Osada1

(1Antibiotics Lab., RIKEN, 2Tokyo Univ. of Sci.)

80 —

P-83 hstD

1 2 2 1 2 (1 2 )

Histone deacetylase sirtuin hstD laeAhstD

LC-Q/TOF-MS hstD 4 RNA

Control ΔhstD 5% 2228 160

PKS NRPSFungiFun FunCat

[secondary metabolism] [detoxification]

hstD 4 SM(methanol : dichloromethane : ethyl acetate=1:2:3 1% formic acid)LC-Q/TOF-MS

hstD

Activation of secondary metabolism in the hstD disruptant of Aspergillus oryzae.

Moriyuki Kawauchi1 2 Rieko Toyoura 2 Kazuhiro Iwashita1 2

(1 Grad. Sch. Adv. Sci. Mat., Hiroshima Univ., 2 NRIB)

P-84 Aspergillus oryzae III CsyB

3-acetyl-6-alkyl- α-pyrone

1 1 1 1 2 1

III CsyB A. oryzae3-acetyl-α-pyrone csypyrone B (CPB) CPB fatty acyl-CoA

pre CPBin vitro

csyB pET22b BL21(DE3) HPLC C15

6 pre CPB in vitro pColdTF Ni-NTA

butyryl-CoA malonyl-CoA acetoacetyl-CoA C3pre CPB (pre CPB-C3) hexanoyl-CoA

octanoyl-CoA pre CPB-C5 -C7CsyB fatty acyl-CoA malonyl-CoA acetoacetyl-CoA 3-acetyl-6-alkyl-α-pyrone

Production of 3-acetyl-6-alkyl-α-pyrones by a type III polyketide synthase CsyB from Aspergillus oryzae

Makoto Hashimoto1, Hiroaki Takahashi1, Tsukasa Koen1, Chihiro Suda1, Katsuhiko Kitamoto2, Isao Fujii1

(1School of Pharmacy, Iwate Medical Univ., 2Dept. of Biotechnol., The Univ. of Tokyo)

81 —

P-85 DNA

1 1 1 2 1 (1 2 )

DNA (DSBs) 1 (NHEJ)

DSBs (HR) 18 I-SceI ,

DSBs ,DSBs HR ( 24) DSBs (GT)

, Zinc finger nucleases (ZFNs) DSBs ,

HR GT 1 Scytalone dehydratase (SDH) 2 ZFNs GT

ZFNs SDH 163 bp 412 bp 18 bpZFN 2A

GT 5 %ZFNs 10 HR

,DSBs HR DNA double-strand breaks mediated targeted gene replacement in the rice blast fungus

Tetsuya Younomaru1, Takayuki Arazoe1, Shuichi Ohsato1, Tsutomu Arie2, Shigeru Kuwata1

(1Sch. Agric., Meiji Univ., 2Grad. Sch. Agric. Sci., Tokyo Univ. of Agric. & Tech.)

P-86 Roxithromycin

(Magnaporthe oryzae),

,

, ,14 Roxithromycin(RXM)

, RXM, , RXM

, RXM ,T7 RXM ,CDC27

, ,RXM , ,

RXM ,,RXM ,

RXM ,

A Possible alternative Target of Roxithromycin in Fungi

Mayu Kumasaka, Akira Ishii, Shinji Kamisuki, Kouji Kuramochi, Fumio Sugawara, Megumi Narukawa-Nara,

Takashi Kamakura

(Dept. of Applied Biological Sci., Tokyo Univ. of science)

82 —

P-87 cAMP

,

cAMP

(Cochliobolus heterostrophus)A(PKA)

PKA (PKR)cAMP

(Cyclase associated protein: CAP)CAP (CAP1)

cAMP

CAP1 CAP1

CAP1PKA1 PKA2 PKR

(PDEL PDEH) cAMP

Characterizations of cAMP signaling pathway associated genes of Cochliobolus heterostrophus

Satoshi Yutani, Takuya Sumita, Yuuki Kitade, Kousuke Izumitsu, Chihiro Tanaka

(Grad. School of Agriculture, Kyoto Univ. )

P-88 Aspergillus fumigatus

,

A. fumigatusA. fumigatus

A. fumigatus

A. fumigatus

dectin1 TLR4

Functional analysis of pathogen related factor in Aspergillus fumigatus.

Kanae Sakai, Motoko Oarada, Tohru Gonoi

(MMRC, Univ. of Chiba)

83 —

P-89 A. fumigatus

1 2 3 2 1 2 3 Aspergillus fumigatus

A. fumigatus

A. fumigatus 44 ( )2 RNA 4

(illumina Miseq) 1. partitivirus 2. chrysovirus 2 3. 4.

A. fumigatus

Isolation and characterization of four types of Mycoviruses in opportunistic pathogen Aspergillus fumigatus Misa Yahara1, Azusa Takahashi-Nakaguchi2, Hiromitu Moriyama3, Tohru Gonoi2 (1Grad. School of Medical and Pharm. Sci., Chiba Univ., 2MMRC, Chiba Univ., 3Tokyo Univ. of Agric. & Tech.)

P-90

3 G 1, 2 1 2 1 2

α β γ G

Gα GβγGγ Gβ

G cAMPGγ ΔGγ

Gβ ΔGβ, Nishimura et al., 2003 Gβγ

GFP Gβ GFP: GβGFP: Gβ ΔGγ

Gγ GβΔGγ ΔGβ 0 cAMP 50

ΔGβ ΔGγ ΔGγΔGβ 10%

ΔGγ ΔGβΔGγ ΔGβ Gβγ

Gβγ

Analyses on a heterotrimeric G-protein γ subunit in Magnaporthe oryzae

Shizuka Kazama1, 2 Hisashi Kawasaki1 Marie Nishimura2 1Tokyo Denki Univ. 2NIAS

84 —

P-91 ChSte20 ChCla4

1 2 1 1 1 1 2

Chk1 MAP

Chk1 MAP PAK-likeSte20 Cla4 ChSte20

ChCla4 ChSte20ChCla4

ChSte20 ChCla4ChSte20 ChCla4

ChSte20 ChCla4PAK-like

ChCla4ChSte20

Characterization of ChSte20 and ChCla4 genes in Cochliobolus heterostrophus

Yuki Kitade1, Kosuke Izumitsu2, Takuya Sumita1, Satoshi Yutani1, Chihiro Tanaka1

(1Grad. Sch. of Agriculture, Kyoto Univ., 2Sch.of Environmental Science,Univ. of Shiga Pref.)

P-92 Ras GTPase CoIra1 CoRas2

cAMP CoMekk1-Cmk1 MAPK

Ras GTPase CoIRA1 CoIra1

, Ira1/2 Ras1/2 cAMP , coira1 cAMP, coira1 cAMP ,CoIra1 cAMPCoIRA1 CoRAS2 coras2 ,

, coras2 ,CoRAS2,CoRas2 CoRAS2 RFP ,CoRas2, CoRas2 , CoRAS2

, coira1 CoRas2CoIra1 CoRas2 , CoRas2 CoMekk1-Cmk1

MAPK CoRAS2 CoMEKK1Δcoras2 CoMEKK1 , , Δcomekk1

CoRAS2 ,CoRas2 CoMekk1-Cmk1CoIra1 CoRas2 cAMP CoMekk1-Cmk1

MAPK

Ras GTPase activating protein CoIRA1 is involved in cAMP signaling transduction pathway and

CoMekk1-Cmk1 MAPK pathway as an upstream regulatory factor in Colletotrichum orbiculare.

Ken Harata Yasuyuki Kubo

(Life and Environmental Science, Univ. of Kyoto Prefectual)

85 —

P-93 MoCV1 MoCV1-B

( ) Magnaporthe oryzae chrysovirus 1 (MoCV1) 5 dsRNA

MoCV1Magnaporthe oryzae chrysovirus 1-B (MoCV1-B) MoCV1

ORF3(dsRNA3 ) ORF4(dsRNA4 ) 83kDa, 85kDa 58kDa, 70kDa MoCV1

MoCV1-B MoCV1MoCV1-B dsRNA1(79.6%), dsRNA2(76.3%), dsRNA3(71.3%), dsRNA4(72.4%), dsRNA5(96.2%) ORF1(92.5%), ORF2(80.1%), ORF3(87.8%), ORF4(89.4%), ORF5(97.1%) ORF2 N ( 200aa) 51.1% ORF3

C ( 200aa) 34.1 CORF1, ORF4 OFR3, ORF4

Comparison of genome structures between Magnaporthe oryzae chrysovirus 1 and Magnaporthe oryzae

chrysovirus 1-B

Ryoko Takai, Syun-ichi Urayama, Hirofumi Sakoda,Yu Katoh, Yuri Kimura, Toshiyuki Fukuhara, Tsutomu Arie,

Tohru Teraoka, Hiromitsu Moriyama (Faculty of Agriculture, Tokyo Univ. of Agri. & Tech)

P-94 Alternaria alternata global regulator LaeA

1 1 2 1 2 3 1 1 2

3 methyltransferase LaeA Aspergillus nidulans global regulator

(Alternaria alternata tomato pathotype, ) LaeA

AaLAEA . (A. alternata strawberry pathotype) (A. alternata apple pathotype) LaeA (KO)

A. alternata 3. KO

LaeA

Functional analysis of the global regulator LaeA homologues in the plant pathogenic Alternaria alternata

Kazumi Takao1, Yasunori Akagi1, Yoshiaki Harimoto2, Toru Ishihara1, Takashi Tsuge2, Eiji Nanba3, Motoichiro

Kodama1

(1Fac. Agric., 3Fac. Medicine, Tottori Univ., 2Grad. Sch. Bioagric. Sci., Nagoya Univ.)

86 —

P-95 BcAtg1

1 2 1 1 2

Botrytis cinerea

Saccharomyces cerevisiaeAtg1 BcAtg1

BcAtg1BcAtg1

BcAtg1BcAtg1

BcAtg1

Characterization of the autophagy-related gene BcAtg1 in Botrytis cinerea

Takuya Sumita1, Kosuke Izumitsu2, Chihiro Tanaka1

(1Grad. Sch. of Agriculture, Kyoto Univ., 2Sch. of Environmental Science, Univ. of Shiga Pref.)

P-96 (O-5) RAM CoPag1

CoKEL2cokel2Δ

CoKEL2 6

RAM (regulation of Ace2p activity and cellular morphogenesis)Pag1 (Tao3) CoPAG1 copag1Δcokel2Δ

CoPAG1CoPag1::RFP

CoPag1::RFP

Colletotrichum orbiculare CoPag1, a component of RAM network in Saccharomyces cerevisiae, is involved in

appressorium development triggered by plant-derived signals

Sayo Kodama, Ayumu Sakaguchi, Yasuyuki Kubo

(Graduate School of Life and Environmental Sciences, Kyoto Prefectural Univ.)

87 —

P-97

1 2 3 4 1

1 2 3 4

(Alternaria alternata tomato pathotype) AALGiberella

AALALT FUM

ALT AALALT 1 Mb conditionally dispensable

chromosome (CDC) CDC CDCCDC optical mapping rare cutting enzyme (NotI)

RFLP (isochromosome) CDC FISH

CDC

Structural analysis of a pathogenicity chromosome in Alternaria alternata tomato pathotype.

Yasunori Akagi1, Masatoki Taga2, Takashi Tsuge3, Eiji Nanba4, Motoichiro Kodama1

(1Fac. Agric., 4Fac. Medicine, Tottori Univ., 2Fac. Sci., Okayama Univ., 3Grad. Sch. Bioagric. Sci., Nagoya Univ.)

P-98 RTS1 CoRTS1

, , ,

, spindle position checkpoint (SPOC) 1

BUB2 CoBUB2 , G1 / S, Bub2 RTS1 CoRTS1 Rts1

SPOC , , rts1RTS1

corts1 , , , ,

, , corts1 CoRTS1::GFP ,

, CoRts1 CoRTS1corts1 , , ,

CoRTS1,

CoRTS1, the homolog of Saccharomyces cerevisiae RTS1 regulating septin dynamics, is required for proper

conidiation and appressorium formation in Colletotrichum orbiculare.

Kou Kawabata, Fumi Fukada, Ayumu Sakaguchi, Yasuyuki Kubo

(Graduate School of Life and Environmental Sciences, Kyoto Prefectural Univ.)

88 —

P-99 Enoyl-CoA hydratase

ATMTColletotrichum orbiculare Path-1 TAIL PCR Path-1

enoyl-CoA hydrataseCoEch1 CoEch1

CoEch1CoEch1 PDA

CoEch1

CoEch1 CoEch1-GFPCoEch1-GFP Mitotracker

Enoyl-CoA hydratase CoEch1 is essential for pathogenicity of Colletotrichum orbiculare

Kosuke Izumitsu, Yasuka Tatara, Kaori Komatsu, Aya Yokoyama, Takuya Taniguchi, Mami Maruyama, Toshikazu

Irie, Kazumi Suzuki (Sch. of Environmental Science, Univ. of Shiga Pref.)

P-100 CoTEA4 CoMOD5

2TEA1 CoKEL2 Tea1

Tea1 Tea4 Mod5CoTEA4 CoMOD5 cotea4

comod5 cotea4cokel2

CoTEA4

Functional analysis of putative cell polarity factors, CoTEA4 and CoMOD5, from Colletotrichum orbiculare

Midori Kawashimo, Ayumu Sakaguchi, Yasuyuki Kubo, Gento Tsuji

(Grad.sch.of Life and Environ. Sci.of Kyoto Prefectural Univ.)

89 —

P-101 terretonin

Aspergillus terreus terretonin farnesyl

terretoninterretonin D

terretonin Aspergillus oryzaeterretonin

terretonin Trt9 short chain dehydrogenase Trt3 FAD-dependent monooxygenase 5

preterretonin A preterrenoidterrenoid

Trt6 P450 Trt7 non-heme iron-dependent dioxygenase Trt14 hypothetical protein

Functional analysis of the tailoring enzymes involved in the biosynthesis of a fungal meroterpenoid terretonin

Taiki Iwabuchi, Yudai Matsuda, Takayoshi Awakawa, Ikuro Abe

(Graduate School of Pharmaceutical Sciences, Univ. of Tokyo)

90 —

----------- B -------------- Ba Van Vu 37 Bernard Henrrissat 40 ----------- C ------------ Chi-Yip Ho 72 ----------- D ------------ Dan Cullen 40 David Hibett 40 Dung Huy Hoang 48 ----------- E ------------ Elisabeth Tillier 72 Emma Master 72 ----------- F ------------ Feng Jie JIN 57 ----------- G ------------ Gerhard H. Braus 53 ----------- J ------------ Jae-Hyuk JANG 79 Jill Gaskel 40 Jong Seog AHN 79 Jun-Pil JANG 79 ----------- K ------------ Kenneth Bruno 48 Kieu Pham Thi Minh 37 Kin Chan 71 ----------- N ------------ Nuo Li 74 ----------- O ------------ Özgür Bayram 53 Özlem Sarikaya Bayram 53 ----------- P ------------ Pei HAN 57 Philip Wong 72 ----------- Q ------------ Quoc Bao Nguyen 37 ----------- S ------------ Scott Baker 48 ----------- Y ------------ Yunchen Gong 72 ----------- ------------

70, 74 5, 87

43

41 39, 41, 44, 46,

47, 61, 67 74, 77, 78a,78b,

89 62

65 32, 66 61 81

81, 85 75, 77, 78a, 78b,

89 34

46 ----------- ------------

14, 40, 41, 68

29,37, 55 81

48 65 42, 71, 82, 84,

86, 88 41

30 66

56 73

85 33, 62 34 75 69 34, 42 45

46, 47 29, 55

37 71, 88 38 30, 35, 44, 52,

77, 80 89

----------- ------------ 44 65 67

43 48 44 85 29, 55

----------- ------------

64

32, 40 ----------- ------------

47 49

82 57

81 33, 60 63

35 38

52, 59 45

68 64

31, 33, 60, 70 41 18

62, 63 46

43 21

34 64 76a, 76b, 79a,

79b 52, 59

35, 44 46

----------- ------------

43 83 68

45 53 67

79 79 59, 60, 69

85 52, 59 69 29, 55 70, 73a, 73b, 74 79, 81 58 81

34 54

44 30, 77, 79 41, 47 55 71, 74

83

88 73 29, 56

87 74

38 ----------- -------