第十二章 医药工业的基因工程Genetic Engineering in Medicine

and Industry

Some therapeutic products for used in humans

Conversion of preproinsulin to insulin

The structure of insulin

Proinsulin and production Proinsulin and production

of recombinant-derivedof recombinant-derived

insulin by separate insulin by separate

fermentations for fermentations for

A- and B- chainsA- and B- chains

Process used to make human insulan from fusion proteins synthesized in E.coli

Expression of humanExpression of human

insulin in insulin in E.coliE.coli

Dwarfism

Human growth hormone deficiency

Structure of the first vector used to produce human growth hormone (hGH) in E.coli

Bacterial Bacterial

production of production of

human growthhuman growth

hormone (hGH)hormone (hGH)

Bacterial Bacterial

production of production of

human growthhuman growth

hormone (hGH)hormone (hGH)

Production of Production of

a subunit vaccine a subunit vaccine

in yeastin yeast

Production of Production of

a subunit vaccine a subunit vaccine

in yeastin yeast

Production of tissue Production of tissue

plasminogen activator plasminogen activator

(tPA) by mammalian (tPA) by mammalian

cell culturecell culture

Production of a Production of a

monoclonal antibody monoclonal antibody

(MAb)(MAb)

Creating a Creating a

combinatorial library combinatorial library

of antibodies of antibodies

expressed in expressed in E.coliE.coli

Direct cloning of Direct cloning of

antibody cDNAs antibody cDNAs

by PCRby PCR

Antibody engineering

A bispecific antibodyA bispecific antibody

Expressions of proteins Expressions of proteins

and peptides on the surface and peptides on the surface

of filamentous phageof filamentous phage

Plants as bioreactors Plants as bioreactors

to produce antibodiesto produce antibodies