curriculum vitae - cairo universityscholar.cu.edu.eg/drrehabhafez/files/dr_rehab_hafez_cv.pdf ·...
TRANSCRIPT
1
CURRICULUM VITAE
PERSONAL DATA:
Name : Rehab Mahmoud Hafez Mahmoud Abdel-Rahman
Profession : Assistant professor, Cytology and Genetics Division,
Botany and Microbiology Department,
Faculty of Science, Cairo University
Address Work : Botany Department, Faculty of Science, Cairo University,
Giza, Egypt
E-mail Official : [email protected]
Others : [email protected]
Phone Office : (+2) 3566671 (+2) 35676644
Mobile : (+2) 01006763263 (+2) 01203770993
Faculty official site: : scholar.cu.edu.eg/drrehabhafez
LANGUAGES:
Arabic (Mother Tongue)
English (Excellent spoken, read & written, National TOEFL in 2000)
French (Very good spoken, read & written)
COMPUTER SKILLS:
Very Good experience in computer common programs: Microsoft Office (Word, Excel
and Power Point) and Photoshop.
Basic IT Skills and Internet course (100 hours), Computers and Information
Technology, Scientific Computation Center, Cairo University (11 June - 13 July 2006).
Good experience in many computer programs for statistical analysis (eg: SPSS).
2
OCCUPATIONS HISTORY:
Demonstrator (1/1997- 6/2006) - Botany and Microbiology Department - Faculty of
Science - Cairo University.
Assistant Lecturer (6/2006 -1/2013) - Botany and Microbiology Department - Faculty
of Science - Cairo University.
Assistant Professor (1/2013 till now) - Botany and Microbiology Department - Faculty
of Science - Cairo University.
EDUCATIONAL BACKGROUND:
B.Sc., 1996 (Botany; Very Good), Faculty of Science, Cairo University.
M.Sc., Jun, 2006 (Botany; Cytology, Genetics and Plant Biotechnology), Faculty of
Science, Cairo University, Egypt.
Titled: Production of solanum elaeagnifolium alkaloids ‘in vitro’ using plant tissue
culture techniques.
Abstract:
The present investigation was conducted to produce alkaloids of Solanum
elaeagnifolium using tissue culture techniques, which included: hairy roots cultures using
Agrobacterium rhizogenes strain ATCC 15834, callus cultures and regenerants.
The highest values of total steroidal alkaloids (TSA) (32.62 mg / g DW) and solasodine
(18.98 mg / g DW) were found in the fruit of the wild plant; much lower values were
recorded in other organs. Diosgenin was detected only in the roots (0.121 mg / g DW).
High values of TSA (10 mg / g DW) and solasodine (7.924 mg / g DW) were produced
from hairy roots developed on shoot explants infected with half strength MS medium
supplemented with bacteria and 10 ml acetosyringone. Diosgenin level was about 0.4
mg / g DW. Callus induction was performed using M2 medium (MS + 0.5 mg / L 2,4-D +
1.0 mg / L KIN) for root (R), hypocotyl (Hy), stem (St) and leaf (L) explants.
Effect of abiotic stresses (sodium chloride and mannitol); biotic stress (Aspergillus niger
extract) and some additives (cholesterol and reduced glutathione) were studied.
3
Among callus cultures, the highest production of TSA (9.6 mg / g DW) was recorded in
St- and Hy-calli treated with 15 mM cholesterol, followed by (8.978 or 8.224 mg / g DW)
in R-calli treated with 40 % fungal extract or 0.05 M NaCl, respectively. Then came
L- and R-calli treated with 0.3 mM GSH (5.952 and 5.503 mg / g DW) and Hy-calli
treated with 0.11 M mannitol (5.032 mg / g DW). Regarding solasodine, St- and Hy-calli
treated with 15 mM cholesterol gave high values (4.582 and 4.265 mg / g DW,
respectively). Regarding diosgenin, the highest production (7.744 mg / g DW) was in
R-calli treated with 40 % fungal extract, followed by St-calli treated with 0.05 M NaCl
(3.049 mg / g DW). Regenerated shoots derived from apical shoot meristem contained
2.619 mg / g DW of TSA, which included equal amounts of solasodine and diosgenin
(about 0.55 mg / g DW). Regenerated plantlet and rooted-calli derived from
Hy-explants showed slight variation of TSA (2.429 and 2.835 mg / g DW, respectively).
The value of solasodine was higher than diosgenin in case of regenerated plantlet, while
the reverse was obtained in case of rooted-calli.
Ph.D., July, 2012 (Botany, Cytology, Genetics and Plant Biotechnology), Faculty of
Science, Cairo University, Egypt.
Titled: Improvement of fungal disease resistance in potato using Defensin gene and in
vitro selection.
Abstract:
Pathogenicity test showed that potato plants were highly susceptible to pathogenic
fungi Rhizoctonia solani, Fusarium dimerum, F. oxysporum, Cladosporium herbarum
and Nigrospora sphaerica. The maximum number of harvested tubers was found in case
of R. solani, while heavier tubers were in case of F. dimerum, F. oxysporum, N.
sphaerica and C. herbarum. The maximum number of tuber eyes was obtained in case of
C. herbarum. Nigrospora sphaerica and C. herbarum expressed wide variation in shapes,
colors and texture of tubers. The light microscopic observations showed changes in the
uppermost coat thickness and texture in all infected tissues compared with control.
Hypodermal tissue of infected tubers showed either hyperplasia in case of R. solani and
4
F. dimerum or hypertrophy in case of F. oxysporum and C. herbarum. Most of the
infected tissues showed the presence of many cytoplasmic vacuoles in hypodermal and
cortical layers with the presence of few starch grains in the cortical cells compared to
those present in the non-infected tuber. Transmission electron microscopy investigations
showed cell wall and cytoplasmic changes and/or degradation, together with membrane
abnormalities. Most of the infected cells showed the deposition of densely opaque
granular material along the plasma membrane and/or tonoplast in addition to dark
inclusions and crystals. Microtuberization reached about 94% on Islam et al. medium
after 10 days. Multiple shoot regeneration from tuber discs (6.1±1.9) were enhanced on
Jarret et al. medium while Yee et al. medium was more efficient in case of leaf explants
(2.6±1.2) so; both media and explants were used in transformation experiments. RAPD
analysis was used to detect similarities or dissimilarities between the normal in vitro
plantlet and the regenerants. The in vitro plantlets were used as source of explants for
transformation and in vitro selection experiments. The tuber discs and leaf explants were
co-cultivated with Agrobacterium tumefaciens on regeneration medium supplemented
with acetoseryngone for 2 days then transfer to selection medium. The putative lines and
control plantlets were subjected histochemical analysis and specific PCR to confirm the
insertion of defensin gene and Gus reporter gene in them. In vitro selection was carried
out and only nodal cuttings exposed to successive rising of the concentration of the N.
sphaerica and C. herbarum exhibited resistance. In vitro plantlets, selected and
transformed lines were acclimatized. Resistance bioassay showed absence of symptoms
on transformed organs and some mild symptoms on the foliar lamina, tubers and calli of
the selected lines when exposed to N. sphaerica and C. herbarum infection units,
compared to those of in vitro plantlet. In vivo test showed that in vitro plants became
wilted with fragile leaves when exposed to R. solani, F. dimerum or F. oxysporum. In
case of N. sphaerica, no symptoms were seen, while in case of C. herbarum, the leaves
showed necrosis spots. Highest reduction in the external symptoms was observed in
transformed lines compared to the in vitro selected plantlets and in vitro plantlets.
5
SCIENTIFIC TRAINING COURSES ATTENDED:
WORKSHOPS in FACULTY and LEADERSHIP DEVELOPMENT
CENTER (FLDC):
Modern Methods in Plant Molecular Biology and Biochemistry,
provided by Plant Cell & Tissue Culture Department, Genetic
Engineering and Biotechnology Division, National Research
Center and Agricultural Analysis unit, Faculty of Agriculture,
Cairo University.
1998 March
Microanalysis and New Technology, Micro Analytical Center,
Faculty of Science, Cairo University. 2004 April
Molecular Biology Training Course, Development of Molecular
Biology Courses Project, Higher Education Enhancement Project
Fund (HEEPF), Faculty of Science, Cairo University.
2006 November
Molecular Genetics Approaches: Principles and Applications,
Molecular Biology Lab, Research Park (CURP), Faculty of
Agriculture, Cairo University.
4201 February
Gene Expression Analysis on Large Scale Using Microarray and
Real Time PCR Techniques, Molecular Biology Lab, Research
Park (CURP), Faculty of Agriculture, Cairo University.
4201 September
Thinking Skills 2006 July
New Trends in Teaching 0620 August
Quality Standards in Teaching 0920 May
Research Ethics 0920 December
International Scientific Publications 1020 May
Student Evaluation 1120 February
Effective Presentation 1201 June
Communication Skills 2201 January
6
OTHER WORKSHOPS
Competing for Research Funds 2013 January
The Credit Hour Systems 2013 January
Conference Organization 2013 May
University Code of Ethics 2013 September
Legal and Financial Aspects in University Environment 2014 January
Managing Research Teams 2014 June
Effective Teaching Skills 2015 April
Statistical Analysis of Bio-Experiments 2017 March
Workshop on How to write proposal provided by Central Lab,
Ebn Sinai Hall, Faculty of Science, Cairo University. 2008 March
Training in course report and program provided by the Quality
Assurance and Accreditation Center, Ebn Sinai Hall, Faculty
of Science, Cairo University.
2011 January
Training in Key Performance Indicators- Ebn Sinai Hall,
Faculty of Science, Cairo University. 2015 November
Training in statistical methods- Ebn Sinai Hall, Faculty of
Science, Cairo University. 2015 November
Training in the principals of ISO 9001/2008 certification -Ebn
Sinai Hall, Faculty of Science, Cairo University. 2016 February
Training in academic advising - Cairo University Centre for
Quality Assurance of Education. 2017 January
Specification of the examination paper and the formulation of
the examination questions - Cairo University Centre for
Quality Assurance of Education.
2017 March
Preparing the specifications of the exam questions (blue 2017 March
7
FIELDS OF INTERST:
Genetics, Cytology, Plant Tissue Culture, Transformation, Molecular Biology,
Mutations, Bioinformatics and Bio-Nanotechnology
TEACHING EXPERIENCES:
For Undergraduate Students:
Botany Courses: Genetics, Anatomy, Morphology, Physiology, Systematic, Ecology, Archegoniate,
Cell Biology, Phycology and Genetic Engineering in plants.
Microbiology courses General Microbiology, Environmental Microbiology, Mycology, Biotechnology,
Microbial enzymes, Virology, Biodiversity, Biological Control and Plant
Pathology.
For Postgraduate students (Applied Microbiology Diplomat):
Virology, Phycology and Environmental microbiology.
LABORATORY and TECHNICAL EXPERIENCES
Expert in techniques used to measure different physiological and biochemical
parameters such as Photosynthetic Pigments, Carbohydrates, Nucleic Acids, Protein
and Glycoalkaloids.
Expert in measuring the activity of some microbial enzymes.
Have good experience in preparation of samples for examination of cell divisions and
chromosomal abnormalities, and analyzing the observed phenomena.
Have some experience in how to prepare samples for TEM and analyzing the
observed phenomena.
print) - Cairo University Centre for Quality Assurance of
Education.
Measurement criteria for practical exams- Cairo University
Centre for Quality Assurance of Education.
2017 March
8
Expert in Tissue Culture (Micropropagation, Callus Cultures, Cell Suspension, and
Organogenesis), in vitro selection and Agrobacterium-mediated transformation
Protocols.
Have good experience in Molecular Biology Protocols.
Have good experience in utilization of some bioinformatics tools.
Expert in isolation and culturing microorganisms (bacteria, fungi and actinomycetes)
and microalgae.
Have good experience in performing mutations of microbes and analyzing the
observed phenomena.
Have good experience in biofuel production using mechanical, chemical and
enzymatic pretreatments.
Proper utilization and maintenance of scientific instruments (PCR, Gel
Electrophoresis, Laminar Flow, Spectrophotometer, Autoclave, Digital Electrical
Balance, Cooling Centrifuge, pH meter, …etc..
PUBLICATIONS:
Mahmoud M. Saker, Salwa A. Abdel-Maksoud and Rehab M. Hafez (2006). In vitro
production of solasodine and diosgenin from hairy root cultures of Solanum
elaeagnifolium, In: 1st International Egyptian-Jordanian conference, p. 160 (abstract)
Mahmoud M. Saker, Salwa A. Abdel-Maksoud and Rehab M. Hafez (2006). Elicitation
of solasodine and diosgenin accumulation in callus cultures of Solanum
elaeagnifolium by Aspergillus niger culture filtrate, In: 1st International Egyptian-
Jordanian conference, p. 161 (abstract)
Salwa A. Abdel-Maksoud, Rehab M. Hafez, Mahmoud M. Saker and Mohamed K. El-
Bahr (2006). Effect of chemical additives on the production of steroidal alkaloids in
callus cultures of Solanum elaeagnifolium, In: 1st International Egyptian-Jordanian
conference, p. 156 (abstract)
Rehab M. Hafez, Salwa A. Abdel-Maksoud, Mahmoud M. Saker and El-Husseiny
Youssef (2006). Effect of salt and osmotic stresses on steroidal alkaloids content of
9
Solanum elaeagnifolium callus cultures, In: 1st International Egyptian-Jordanian
conference, p. 157 (abstract)
Mahmoud M. Sakr, Amany H. A. Abo ELLil, Tarek A. A. Moussa, Rehab M. H. Abdel-
Rahman (2009). Development of Efficient regeneration System for Potato
Transformation, In: 3rd
International conference of Genetic Engineering and
Biotechnology Research Division (abstract).
Tarek A. A. Moussa, Mahmoud M.M. Saker, Nahed Z.M. Heikal, Amany H. A. Abo
ELLil, Rehab M. H. Abdel-Rahman (2010). Efficient transformation system for
fungal disease resistance in potato, In: 1st International Biotechnology Innovation
Conference, p. 51 (abstract).
Mahmoud M. Saker, Tarek A. A. Moussa, Nahed Z. Heikal, Amany H. A. Abo ELLil
and Rehab M. H. Abdel-Rahman (2012): Selection of an efficient in vitro
micropropagation and regeneration system for potato (Solanum tuberosum L.) cultivar
Desirée . African Journal of Biotechnology 11(98): 16388-16404.
SUPERVISION:
PhD Thesis (since 2015) in Microbiology, Microbiology Department, Faculty of science,
Ain Shams University.
MSc Thesis (since 2016) in Microbiology, Botany and Microbiology Department,
Faculty of science, Cairo University.
MSc Thesis (since 2016) in Cytology and Genetics, Botany and Microbiology
Department, Faculty of science, Cairo University.
MSc Thesis (since 2017) in Microbiology, Botany and Microbiology Department,
Faculty of science, Cairo University.
MEMBERSHIP OF SCIENTIFIC SOCIETIES:
1. Member in the Egyptian Botanical Society, Egypt
2. Member in the Egyptian Society of Experimental Biology, Egypt.
10
CONFERENCES’ ATTENDENCE:
December 2006 : Participation in the 1st International Egyptian-Jordanian
Conference under the theme of "Biotechnology and
sustainable development: Current Status and Future
Scenarios", National Research Center, Cairo, Egypt
November 2009 : Participation in the 3rd
International conference of Genetic
Engineering and Biotechnology Research Division under the
theme of "Biotechnology For Better Life", National Research
Center, Cairo, Egypt
November 2010 : Participation in the 1st International Biotechnology Innovation
Conference (IBIC-Egypt 2010), Faculty of Science, Cairo
University, Cairo, Egypt
April 2011 : Attendance in the 7th
Annual International Conference of
Egyptian Society of Experimental Biology (ESEB-Egypt
2011), Faculty of Science, Cairo University, Cairo, Egypt
July 2011 : Attendance of the 2nd
International Workshop on Industrial
Biotechnology, Conference Center, Cairo University
April 2016 : Attendance in the 1st International Conference of Genetic
Engineering and Biotechnology: Integrative Science and
Biotechnology, Genetic Engineering and Biotechnology
Research Institute (GEBRI), University of Sadat City, Sharm
El Sheikh, Egypt
OTHER ACTIVITIES:
1. Member of Questionnaires Unit in Botany Department, Faculty of Science, Cairo
University, from 2004 to 2014.
11
2. Performing poster and flier for Botany Department (2009) about its history and
sections, with Prof. Dr. Effat Shabana (Head of department) and revised by Prof. Dr.
Mohamed Ibrahim.
3. Performing flier for Botany Department (2012) about the pioneer and elites of our
Departments and the different work facilities of our graduates, with Prof. Dr. Tahany
Abdel-Rahman and other colleagues.
4. Member in the Postgraduates Tables Committee (Diploma, MSc, PhD) in Botany
Department from 2012 to 2015.
5. Executive Director of the Questionnaires Unit, Quality Assurance Unit, Faculty of
Science, Cairo University (2014-2015).
6. Member of Quality Assurance Unit in Botany Department, Faculty of Science, Cairo
University, (2006 to 2015): performing the courses’ specification and reports of the
postgraduate program in each term.
7. Work as general academic adviser for undergraduate students of the Faculty of
Science (2016).
8. Course specifications and course reports for undergraduate courses from 2012 till
now.
9. Member in some Committees in Botany Department (Library, Computer, Books and
Notes publications, from 2013 till now
10. Director of Questionnaires Unit, Quality Assurance Unit, Faculty of Science, Cairo
University, from 2015 till now.
11. Responsible of Quality Assurance Unit for Postgrades affairs in Botany Department,
Faculty of Science, Cairo University, from 2015 till now.
12. Work as a four year academic adviser for 10 undergraduate students of Biotechnology
program, from 2017 till now.
12
AWARDS
- Certificate of Appreciation being the best assistant lecturer in Cairo university (2010).
- Certificate of Appreciation (6-2011) for outstanding performance from the Botany
Department, Faculty of Science, Cairo University.
- Certificate of Appreciation (4-2016) for outstanding efforts in enriching the figures of the
graduates in 2016, Faculty of Science, Cairo University.
ACADEMIC REFEREE
Prof. Dr. Abdel-Fattah Badr (Professor of Plant Genetics)
Botany & Microbiology Department, Faculty of Science, Helwan University, Cairo,
Egypt
e-mail: [email protected]
Prof. Dr. Mahmoud Sakr (Professor of Plant Biotechnology)
President of Academy of Scientific Research & Technology (ASRT), Kasr Al-Eini,
Cairo, Egypt.
e-mail: [email protected]
Prof. Dr. Tarek Abdel-Mawgoud Moussa (Professor of Microbiology)
Botany & Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt.
e-mail: [email protected]
Prof. Dr. Tahany Abdel-Rahman (Professor of Microbiology)
Botany & Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt.
e-mail: [email protected]
Prof. Dr. Maimouna Kord (Professor of Plant Physiology)
Botany & Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt.
e-mail: [email protected]
Prof. Dr. Hasnaa Hosni (Professor of Taxonomy)
Botany & Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt.
e-mail: [email protected]