lab exercise 14:
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Lab Exercise 14:. Biochemical Differentiation of some Medically Important Gram Positive Cocci. Beta ( β ) hemolysis (blood agar around bacterial colonies is completely clear, indicating complete breakdown and consumption of hemoglobin). - PowerPoint PPT PresentationTRANSCRIPT
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Lab Exercise 14: Biochemical Differentiation of some Medically Important Gram Positive
Cocci
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Beta ( β ) hemolysis (blood agar around bacterial colonies is completely clear, indicating complete breakdown and
consumption of hemoglobin)
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Alpha (α) hemolysis (blood agar around bacterial colonies looks greenish-brown, indicating partial breakdown and consumption of hemoglobin)
Mixed culture from a throat swab; note several β-hemolytic colonies
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Gamma (γ) hemolysis (really means no hemolysis; blood agar around colonies looks normal)
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Side-by-side comparisons of alpha (α), beta (β), and gamma (γ) hemolysis
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Bacitracin (Taxo A) sensitivity
+ results with a zone of inhibition
A
- Result with no zone of inhibition
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Optochin (Taxo P) sensitivity
- Results with no zone of inhibition + results with a zone of inhibition
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PP
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Carbohydrate Fermentation Tests (Glucose and Mannitol)
+ - (same as uninoculated media)
Phenol red indicator turns yellow at acid pH
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Catalase Test
- +
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Staphylococcus epidermidis
Enterococcus faecalis
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G M
Who am I?
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!
Answer: Streptococcus pyogenes
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G M
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!
Answer: Micrococcus lutea
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G M
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!
A
Answer: Enterococcus faecalis
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G M
Use the chart on p. 146 and or the flow chart on p. 147 of your lab manual to figure out who I am!
Answer: Staphylococcus aureus
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M G
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!
Answer: Staphylococcus epidermidis
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M G
Use the chart on p. 146 and or the flow chart on p. 147 of your lab manual to figure out who I am!
Answer: Streptococcus pneumoniae
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G M
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!
A
Answer: Streptococcus mutans