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vkbZ0 ts0 flag ,oa vthr flag

ekfRL;dh thofoKku foHkkxeRL; foKku egkfo|ky;Xksk0 c0 iar f"k ,oa izkS|ksfxdh egkfo|ky;]

iUruxj m/ke flag uxj mkjkapy

vkuqoafkd laj{k.k ,oa kvkuqoafkd laj{k.k ,oa kfrd tyfrd tyiz{ks=ksa esa la[;k o`f) gsrq nks khriz{ks=ksa esa la[;k o`f) gsrq nks khr

tyh; eNfy;ksa, dkyh jksgw rFkk eksg, dstyh; eNfy;ksa, dkyh jksgw rFkk eksg, dsiztudksa dk rkykc ds c/ks ty eas ikyuiztudksa dk rkykc ds c/ks ty eas ikyu

,oa cht mRiknu,oa cht mRiknu

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FishFish Labeo dyocheilusLabeo dyocheilus andandLabeo deroLabeo dero

BothBoth areare importantimportant foodfood fishfish inhibitinginhibiting uplanduplandstreamsstreams andand riversrivers atat aa meanmean seasea levellevel (MSL)(MSL) ofof400400--800800mm inin lowerlower HimalayanHimalayan regionregion ofofUttaranchalUttaranchal

LocallyLocallyLL.. dyocheilusdyocheilus isis knownknown asas Kali,Kali, KaliKali rohurohuoror KhaddKhadd andandLL.. derodero isis knownknown asas MohMoh

OrderOrder -- CypriniformesCypriniformes

FamilyFamily -- CyprinidaeCyprinidae

SubSub familyfamily CyprininaeCyprininae

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Sta

tusS

ta

tus

Vulnerable fish Dubey, 1994 and Prasad, 1994Vulnerable fish Dubey, 1994 and Prasad, 1994

Vulnerable fishVulnerable fish -- Perspective Plan DocumentPerspective Plan Document

(VISION 2020) of National Bureau of Fish(VISION 2020) of National Bureau of FishGenetic Resources (NBFGR), LucknowGenetic Resources (NBFGR), Lucknow

Threatened Desai, 1994Threatened Desai, 1994

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Reasons

for l

oss

offish p

opula

ti

onsReas

ons

for l

oss

offish p

opula

ti

ons

Indiscriminate exploitationIndiscriminate exploitation

Habitat lossHabitat loss

PollutionPollution

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St

rat

egiesfo

rf

isheries management

St

rat

egiesfo

rf

isheries management

Catch regulationCatch regulation

Habitat restorationHabitat restoration

Stock supplementationStock supplementation

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Co

nservat

io

n st

rat

egiesCo

nservat

io

n st

rat

egies

In-Situ in natural habitat

Ex-Situ

1. Cryopreservation

2. Maintaining stock in captivity

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ObjectiveObjective

Collection of broodstockfrom nature & rearing inCollection of broodstockfrom nature & rearing in

captivity tofull maturitycaptivity tofull maturity

Seed production through induced breeding inSeed production through induced breeding in

captivitycaptivity

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Collection SiteCollection Site

The adult specimens ofThe adult specimens ofL. dyocheilusL. dyocheilus andandL. deroL. dero

were collected from Marchula, Domunda (Ramwere collected from Marchula, Domunda (Ram

ganga river) and Kosi barrage, Tiger top (Kosiganga river) and Kosi barrage, Tiger top (Kosiriver)river)

Collection year 2000Collection year 2000-- 20042004

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River bed KosiRiver bed Kosi

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Ram Nagar BarrageRam Nagar Barrage

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Rearing and FeedingRearing and Feeding

Adults collected from nature were brought in livecondition and were reared in earthen ponds atCollege of Fisheries, Pantnagar

Regular water supplementation through borewellwater feeding

Feeding with conventional fish feed (oilcake andrice polish, 1:1) @ 5% body weight, twice daily

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Observation on GSI, CF and SCF of adultObservation on GSI, CF and SCF of adultL. dyocheilusL. dyocheilus

collected from wild during 2003collected from wild during 2003

MonthsMonths ParametersParameters No. ofNo. offishfish

MaleMale No. ofNo. offishfish

FemaleFemale

RangeRange AverageAverage RangeRange AverageAverage

May,May, 20032003 BodyBody weightweight (g)(g) 0404 310310 720720 570570 0404 710710 11001100 787.5787.5

BodyBody lengthlength (cm)(cm) 30.030.0 41.041.0 36.736.7 37.037.0 46.746.7 41.341.3

GonadGonad weightweight (g)(g) 3.1763.176 7.6737.673 4.004.00 23.43023.430 65.78365.783 47.54347.543

GSIGSI 0.8570.857 1.0651.065 0.9970.997 3.0823.082 7.8727.872 6.1756.175

CFCF 10451045 12041204 11191119 10691069 11451145 10991099

SCFSCF 10341034 11941194 11581158 985985 10681068 10311031

June,June, 20032003 BodyBody weightweight (g)(g) 0101 4545 4545 0303 6565 105105 88.388.3

BodyBody lengthlength (cm)(cm) 14.514.5 14.514.5 17.017.0 22.022.0 20.020.0

GonadGonad weightweight (g)(g) 0.2300.230 0.2300.230 5.2305.230 13.31013.310 9.6129.612

GSIGSI 0.5110.511 0.5110.511 8.5558.555 12.67612.676 10.69810.698

CFCF 14761476 14761476 986986 13231323 11121112

SCFSCF 14691469 14691469 861861 12101210 995995

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Data on GSI, CF and SCF of adultData on GSI, CF and SCF of adultL. deroL. dero collected from naturecollected from nature

in 2003in 2003

MonthsMonths ParametersParameters No.No.

ofoffishfish

MaleMale No. ofNo. of

fishfish

FemaleFemale

RangeRange AverageAverage RangeRange AverageAverage

May,May,

20032003

BodyBody weightweight (g)(g) 0202 650650 760760 705705 0202 710710 730730 720720

BodyBody lengthlength (cm)(cm) 39.039.0 42.042.0 41.041.0 41.041.0 42.042.0 41.541.5

GonadGonad weightweight (g)(g) 6.6936.693 7.1177.117 6.9056.905 88.97688.976 90.08890.088 89.53289.532

GSIGSI 0.9360.936 1.0291.029 0.9820.982 12.18812.188 12.68812.688 12.43812.438

CFCF 10261026 10961096 10611061 958958 10591059 10091009

SCFSCF 10161016 10841084 10501050 837837 930930 883883

June,June,

20032003

BodyBody weightweight (g)(g) 0101 500500 500500 0404 340340 625625 493.7493.7

Body

Body leng

thleng

th (cm)(cm)

39.

039.

0 39.

039.

0 32.

0

32.

0

37.

037.

0 34.634.6

GonadGonad weightweight (g)(g) 7.9417.941 7.9417.941 46.17246.172 74.13174.131 64.57264.572

GSIGSI 1.5881.588 1.5881.588 11.86011.860 14.41214.412 13.21513.215

CFCF 843843 843843 10381038 12341234 11691169

SCFSCF 830830 830830 897897 10881088 10141014

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Br

ooders

of

Br

ooders

ofL. dyocheilusL. dyocheilus

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Broodstock selection & AcclimatizationBroodstock selection & Acclimatization

MaleMale Rough Pectoral fin & oozing out miltRough Pectoral fin & oozing out milt

after gentle pressure on bellyafter gentle pressure on belly

FemaleFemale Pectoral fin soft, bulging & softPectoral fin soft, bulging & soft

bellybelly

Brooders acclimatized for 3Brooders acclimatized for 3--4 hrs before induction4 hrs before induction

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Induced Breeding ofInduced Breeding ofL. dyocheilusL. dyocheilus andandL. deroL. dero

Inducing hormoneInducing hormone -- OvaprimOvaprim

Induced breeding trials were conducted in circularInduced breeding trials were conducted in circularhatchery at college of Fisheries, Pantnagarhatchery at college of Fisheries, Pantnagar

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Details of induced breeding trials ofDetails of induced breeding trials ofL. dyocheilusL. dyocheilus

S.

No.

Sex Range of

Weightof

fish (g)

Dose of

ovaprim

(ml/kg)

Total No.

ofeggs

released

(Lakh)

Range of

ova dia

(mm)

Avg. dia

ofeggs

(mm)

Fertilization

(%)

Hatching

(%)

Water

temperature

(0C)

Breeding trial inYear 2001

1. Female 600- 1000 0.6 4.30 3.0-5.0 4.1 95.0 97% 25.0-28.5

2. Male 400- 950 0.4 & 05

Breeding trials in year 2002

1. Female 1200- 1250 0.6 & 0.7 6.25 2.7-4.8 4.6 84.0 62.0 24.5 33.0

2. Male 750- 1000 0.4

Breeding trials in Year 2003

1. Female 450- 1350 0.6 & 0.7 6.29 3.6-4.3 4.0 76.3 69.7 28.032.5

2. Male 750 0.4

Breeding trials in 2004

1. Female 500- 1350 0.6 4.81 2.9 4.1 3.4 91.4 76.0 24.5 26.0

4. Male 300-1000 0.4

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Details of Latency period, egg released,Details of Latency period, egg released,

Hatching & Yolk resorption time inHatching & Yolk resorption time in

different breeding trials ofdifferent breeding trials ofL. dyocheilusL. dyocheilus

Year/ Trial Latency

period (hrs)

Number of

egg/kg fish

Average

hatching Time

(hrs)

Yolk sac

Absorption

(hrs)

2001 1st 7.30 268750 15.00 60-62

2002 1st 8.00 255000 14.30

2002 2nd 7.30 255488 12.45

2003, 1st

8.30 97500 15.30 60

2003, 2nd 10.00 259259 15.00

2003, 3rd 9.00 360000 14.00

2004, 1st 9.30 174727 15.00

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Details of induced breeding trials ofDetails of induced breeding trials ofL. deroL. dero

S.

No.

Sex Range of

Weightof

fish (g)

Dose of

ovaprim

(ml/kg)

Total No.

ofeggs

released

(Lakh)

Range of

ova dia

(mm)

Avg. dia

ofeggs

(mm)

Fertilization

(%)

Hatching

(%)

Water

temperature

(0C)

Breeding trial inYear 2002

1. Female 500 0.8 1.71 3.1 4.0 3.9 52.0 64.2 26.0-28.0

2. Male 150- 300 0.4

Breeding trials in year 20031. Female 275 - 600 0.8 & 0.9 3.32 4.8 5.7 5.0 72.2 67.6 25.5 31.5

2. Male 200- 450 0.6 & 0.7

Breeding trials in Year 2004

1. Female 460-480 0.8 & 0.9 2.85 3.2 4.3 3.6 89.2 63.6 25.0 28.5

2. Male 260

-

510 0

.4

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Details of Latency period, egg released, Hatching & YolkDetails of Latency period, egg released, Hatching & Yolk

resorption time in different breeding trials ofresorption time in different breeding trials ofL. deroL. dero

Year/ Trial Latency

period (hrs)

Average hatching

Time (hrs)

Yolk sac

Absorption (hrs)

2002 1st

11 - 17 14.30 58- 64

2003 1st 9.00 15.30

200 2nd 10.30 15.00

2003, 3st 9.30 14.00 56- 63

2004 9.00 13.30

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Mature FemaleMature FemaleL. dyocheilusL. dyocheilus

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Fertilized eggs ofL. dyocheilus

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Comma shape embryoComma shape embryo ofofL. dyocheilusL. dyocheilus

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Startof hatching ofL. dyocheilus

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Hatchling ofL. dyocheilus

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Fingerlings ofFingerlings ofL. dyocheilusL. dyocheilus

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AdultAdultLabeo dyocheilusLabeo dyocheilus

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Fertilized eggs ofFertilized eggs ofL. deroL. dero

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Comma shape embryoComma shape embryo ofofL. deroL. dero

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Startof hatching ofStartof hatching ofL. deroL. dero

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50% hatching of50% hatching ofL. deroL. dero

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One week hatching ofOne week hatching ofL. deroL. dero

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