This report summarizes the interaction of IL22 with 12 transcription factors: STAT03, CSLC, CSLH, CSLL, CMAF01, RORA1, AP1, STAT5A1, NFKappaB1, RORA2, STAT5A4, STAT5B1.

Patser/Conservation scores were generated with the formula:

€

lnnk + pk

N +1 ⎛ ⎝ ⎜

⎞ ⎠ ⎟ 1pk

⎛ ⎝ ⎜

⎞ ⎠ ⎟× ck

⎛

⎝ ⎜

⎞

⎠ ⎟

k =i

i+L

∑ , where i is the

position in the sequence, L is the length of the transcription factor binding site, nk is the kth element of the alignment matrix, pk is the fraction of each base in the genome (for each base, pb=0.25), and ck is the conservation score at position k, and N is the number of sequences being compared. These are the top 3 scores as determined by Patser, with their corresponding Patser/Conservation scores. The conservation scores and raw sequences were obtained from the UCSC Genome Browser. The logos were generated by enoLOGOS at http://www.benoslab.pitt.edu/cgi-bin/enologos/enologos.cgi. These scores were compiled in accordance to the highest Patser/Conservation scores.

STAT03 (12.03)Frequency Matrix: A 20 13 38 6 321 8 6 585 606 191 C 19 10 552 541 21 0 2 21 1 15 G 25 129 9 1 148 605 592 7 5 393 T 549 461 14 65 123 0 13 0 1 14Sequece Logo:

Putative Sites:1)

Site: 117641779 Patser: 3.33

Cons/Pat: 5.37

Mouse GGACTGGAAAHuman GTACTGGAAARat GGACTGGAAADog GTACTGGAAA

Site: 117641765C Patser: 4.84

Cons/Pat: 4.70

Mouse TTCTGAGAAGHuman TTCTGAGAACRat TTCTGAGAAGDog TTCTGAGAAC

Site: 117622444 Patser: 3.06

Cons/Pat: 4.44

Mouse TGCCTGGAAGHuman AGCCAGAAGGRat ==========Dog ==========

CSLC (6.71)A 5 20 21 21 0 0C 2 0 0 0 0 0G 9 0 0 0 21 0T 5 0 0 0 0 21

Site: 117623494 Patser: 6.71

Cons/Pat: 4.50

Mouse TAAAGTHuman TAAAGTRat TAAAGTDog TGAAGT

Site: 117641611C Patser: 2.835

Cons/Pat: 2.877

Mouse GAAAGCHuman GAACACRat GAAAACDog GAAAGC

Site: 117623842C Patser: -1.607

Cons/Pat: 2.819

Mouse GAAAAAHuman GAAATARat GAAAGADog GAAACG

CSLHA 0 0 0 0 7 1 24 24C 14 0 0 0 0 0 0 0G 0 24 0 24 15 23 0 0T 10 0 24 0 2 0 0 0

Site: 117641714 Patser: 5.77

Cons/Pat: 5.47

Mouse GGTGGGAAHuman GGTGGAAARat GGTGGGAADog GGTGGGAA

Site: 117622001 Patser: 5.77

Cons/Pat: 5.47

Mouse GGTGGGAAHuman ==========Rat ==========Dog ==========

Site: 117641766C Patser: 4.16

Cons/Pat: 4.179

Mouse TCTGAGAAHuman TCTGAGAARat TCTGAGAADog TCTGAGAA

CSLLA 10 0 0 7 1 24 10C 2 0 0 0 0 0 2G 10 0 24 15 23 0 10T 2 24 0 2 0 0 2

Site: 117641715 Patser: 7.26

Cons/Pat: 6.33

Mouse GTGGGAAHuman GTGGAAARat GTGGGAADog GTGGGAA

Site: 117641766C Patser: 5.00

Cons/Pat: 5.01

Mouse CTGAGAAHuman CTGAGAARat CTGAGAADog CAGAGAA

Site: 117623542C Patser: 5.75

Cons/Pat: 4.56

Mouse GTGGGATHuman GTGGGATRat GTGGGATDog ATGGGAT

CMAF01Frequency Matrix:A 8 15 11 12 6 3 1 3 3 2 32 2 6 4 10 10 9 7 14C 5 4 5 7 4 2 2 33 2 1 0 23 2 15 5 3 14 5 7G 14 5 6 3 12 0 34 0 2 33 2 5 16 7 12 14 6 10 9T 10 13 15 15 15 32 0 1 30 1 3 7 13 11 10 10 8 15 7

Sequence Logo:

Site: 117641536 Patser: 6.03

Cons/Pat: 6.27

Mouse CAAAACGCTGACTCAATAGHuman CAAAATGCTTACTCAGCCARat CAACATGCTGACTCAGTAGDog CAAACTGTTTACTCAGCTA

Site: 117642214 Patser: 3.41 Cons/Pat: 4.34

Mouse CTTTATGCTGGCCAAGGAGHuman CTTCATGCTGGCTAAGGAGRat CTTTATGCTGGCCAAGGAGDog CTTCATGCTGGCTAAGGAG

Site: 117630786 Patser: 7.54 Cons/Pat: 3.42

Mouse AATGTTGCTGATAAAACTAHuman ==================Rat CATGTTGCTGATAAAACTADog ==================

RORA1A 10 9 15 9 6 11 21 0 0 0 0 25 7C 2 3 1 1 12 2 0 0 0 0 25 0 5G 7 3 2 0 4 5 4 25 25 0 0 0 2T 6 10 7 15 3 7 0 0 0 25 0 0 10

Site: 117623847 Patser: 8.04

Cons/Pat: 7.91

Mouse CAAAAGAGGTCACHuman CCAAAGAGGTTACRat CCAAAGAGGTCATDog CCAAAGAGGTCAC

Site: 117623490 Patser: 2.67

Cons/Pat: 4.21

Mouse AGCTTAAAGTCATHuman AGTTTAAAGTCATRat AGCTTAAAGTCATDog

Site: 117641870 Patser: -10.93

Cons/Pat: 3.07

Mouse CCTCTCCCATCACHuman CCTTCCCCGTCACRat CCTCTCCTATCACDog CCTT -CCCCTCAC

AP1

A 0 0 16 5 3 0 16 C 1 0 2 12 0 15 0 G 0 15 0 1 1 3 1 T 17 3 0 0 14 0 1

Site: 117641544 Patser: 8.13

Cons/Pat: 7.69

Mouse TGACTCAHuman TTACTCARat TGACTCADog TTACTCA

Site: 117641544C Patser: 5.846

Cons/Pat: 5.422

Mouse TGAGTCAHuman TGAGTAARat TGAGTCADog TGAGTAA

Site: 117629136C Patser: 5.13

Cons/Pat: 8.128

Mouse TGACTCAHuman =======Rat TGAGGCADog TAATTTA

STAT5A1

A 7 18 14 0 0 0 0 10 8 0 33 33 13 2 8C 6 2 4 0 1 32 15 6 0 0 0 0 7 11 8G 8 7 4 0 0 1 6 8 25 33 0 0 4 0 6T 7 3 11 33 32 0 12 9 0 0 0 0 8 19 8

Site: 117641763 Patser: 6.41

Cons/Pat: 7.44

Mouse TACTTCTCAGAAGACHuman TCGTTCTCAGAAGACRat TACTTCTCAGAAGGCDog TAGTTCTCAGAAGAC

Site: 117641763C Patser: 7.96

Cons/Pat: 7.24

Mouse GTCTTCTGAGAAGTAHuman GTCTTCTGAGAACGARat GCCTTCTGAGAAGTADog GTCTTCTGAGAACTA

Site: 117641637 Patser: 6.56

Cons/Pat: 4.81

Mouse GATTTCTGGGATTTGHuman AATTTCTGGGATTTGRat GATTTCTGGGATTTGDog AATTTCTGGGATTTA

NFKappaB1

A 0 0 0 2 11 5 0 0 0 0 1 C 0 0 0 0 1 0 5 13 17 18 15G 18 18 18 16 6 2 2 0 0 0 1 T 0 0 0 0 0 11 11 5 1 0 1

Site: 117623536C Patser: 5.973

Cons/Pat: 5.663

Mouse GGGATTTTCCCHuman GGGATT - - - CCRat GGGATT - - - CCDog GGGATT - - - CC

Site: 117623537C Patser: 4.842

Cons/Pat: 5.540

Mouse TGGGATTTTCCHuman TGGGATT - - - CRat TGGGATT - - - CDog TGGGATT - - - C

Site: 117623536 Patser: 5.21

Cons/Pat: 4.92

Mouse GGGAAAATCCCHuman GG - - - AATCCCRat GG - - - AATCCCDog GG - - - AATCCC

RORA2

A 17 15 36 22 2 0 28 0 0 0 0 36 14C 2 0 0 0 12 0 0 0 0 0 36 0 7G 7 4 0 0 13 0 8 36 36 0 0 0 10T 10 17 0 14 9 36 0 0 0 36 0 0 5

Site: 117623847 Patser: 4.01

Cons/Pat: 3.92

Mouse CAAAAGAGGTCACHuman CCAAAGAGGTTACRat CCAAAGAGGTCATDog CCAAAGAGGTCAC

Site: 117642062 Patser: -7.96

Cons/Pat: 3.49

Mouse AAATCTATGAGTTHuman AAATCTGTGAGCTRat AAATCTATGAGCTDog AAATCTGTGAGCT

Site: 117641500 Patser: -8.83

Cons/Pat: 3.45

Mouse GAATCTATGAAGTHuman GAATCTATAAAGCRat GAATCTATGAAGTDog GAATCTATGAAGC

STAT5A4

A 21 40 18 0 0 5 2 29C 32 15 29 0 0 95 35 21G 31 11 18 0 0 0 6 21T 16 34 35 100 100 0 56 29

Site: 117642059C Patser: 5.737

Cons/Pat: 5.420

Mouse GATTTCTGHuman GATTTCTGRat GATTTCCTDog GATTTCTG

Site: 117641783C Patser: 5.203

Cons/Pat: 5.197

Mouse AATTTCCAHuman AATTTCCARat AATTTCCADog AATTTCCA

Site: 117641763 Patser: 4.90

Cons/Pat: 4.90

Mouse TACTTCTCHuman TCGTTCTCRat TACTTCTCDog TAGTTCTC

STAT5B1

A 9 24 16 0 0 0 0 7 10 0 46 46 17 8 8C 6 2 1 0 0 46 28 11 2 0 0 0 5 10 11G 14 8 3 0 0 0 1 8 34 46 0 0 5 1 7T 11 8 26 46 46 0 17 20 0 0 0 0 19 27 17

Site: 117641763C Patser: 5.69

Cons/Pat: 6.78

Mouse GTCTTCTGAGAAGTAHuman GTCTTCTGAGAACGARat GCCTTCTGAGAAGTADog GTCTTCTGAGAACTA

Site: 117641763 Patser: 5.95

Cons/Pat: 6.49

Mouse TACTTCTCAGAAGACHuman TACTTCTCAGAAGGCRat TACTTCTCAGAAGGC Dog TAGTTCTCAGAAGAC

Site: 117641637 Patser: 6.97

Cons/Pat: 5.05

Mouse GATTTCTGGGATTTGHuman AATTTCTGGGATTTGRat GATTTCTGGGATTTGDog AATTTCTGGGATTTA